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NRAMP1/SLC11A1 Antibody (910825)

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Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NRAMP1/SLC11A1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for NRAMP1/SLC11A1 at approximately 90-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NRAMP1/SLC11A1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAMP1/SLC11A1 ...read more
HEK293 human embryonic kidney cell line transfected with either (A) human NRAMP1/SLC11A1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human NRAMP1/SLC11A1 Monoclonal Antibody (Catalog # MAB8400) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
910825
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8400U-100UG
Alexa Fluor 405
FAB8400V-100UG
Alexa Fluor 488
FAB8400G-100UG
Alexa Fluor 532
FAB8400X
Alexa Fluor 594
FAB8400T-100UG
Alexa Fluor 647
FAB8400R-100UG
Alexa Fluor 700
FAB8400N-100UG
Alexa Fluor 750
FAB8400S-100UG
Allophycocyanin
FAB8400A-025
Allophycocyanin/Cy7
FAB8400APCCY7
Biotin
FAB8400B
CoraFluor 1
FAB8400CL1
DyLight 350
FAB8400D
DyLight 405
FAB8400E
DyLight 488
FAB8400K
DyLight 550
FAB8400L
DyLight 594
FAB8400M
DyLight 650
FAB8400W
DyLight 680
FAB8400Y
DyLight 755
FAB8400Z
FITC
FAB8400F
HRP
FAB8400H
Janelia Fluor 525
FAB8400JF525
Janelia Fluor 549
FAB8400I
Janelia Fluor 585
FAB8400JF585
Janelia Fluor 635
FAB8400JF635
Janelia Fluor 646
FAB8400J
Janelia Fluor 669
FAB8400JF669
PE
FAB8400P
PE/Atto594
FAB8400PEATT594
PE/Cy5.5
FAB8400PECY55
PE/Cy7
FAB8400PECY7
PerCP
FAB8400C
mFluor Violet 450 SE
FAB8400MFV450
mFluor Violet 500 SE
FAB8400MFV500
mFluor Violet 610 SE
FAB8400MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB8400A-025
Carrier Free
MAB8400-SP
Carrier Free
FAB8400V-100UG
Carrier Free
FAB8400U-100UG
Carrier Free
FAB8400T-100UG
Carrier Free
FAB8400S-100UG
Carrier Free
FAB8400R-100UG
Carrier Free
FAB8400N-100UG
Carrier Free
FAB8400G-100UG
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NRAMP1/SLC11A1 Antibody (910825) Summary

Immunogen
NS0 mouse myeloma cell line transfected with human NRAMP1/SLC11A1
Met1-Gly550
Accession # NP_000569
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human NRAMP1/SLC11A1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC11A1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NRAMP1/SLC11A1 Antibody (910825)

  • natural resistance-associated macrophage protein 1
  • LSH
  • member 1
  • NRAMP 1
  • NRAMP1
  • NRAMP1solute carrier family 11 (sodium/phosphate symporters), member 1
  • NRAMPnatural resistance-associated macrophage protein 1
  • SLC11A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)

Background

NRAMP1/SLC11A1 (Natural Resistance-Associated Macrophage Protein 1/Solute Carrier family 11, member 1) is a member of the solute carrier family of multi-pass membrane proteins. It functions as a proton-coupled divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in SLC11A1 have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn’s disease. Alternatively spliced variants that encode different protein isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NRAMP1/SLC11A1
Antibody (910825)
MAB8400
NRAMP1/SLC11A1 Antibody (910825)
Species: Hu
Applications: WB, Flow, CyTOF-ready

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Bioinformatics

Gene Symbol SLC11A1
Uniprot