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Serpin F1/PEDF Antibody [Unconjugated]

 (6 Publications)
 
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Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat ...read more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1149
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1149-SP
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Serpin F1/PEDF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Serpin F1/PEDF
Asp43-Thr417
Accession # BAA31978
Specificity
Detects mouse Serpin F1/PEDF in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human Serpin F1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF1149 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin F1/PEDF Antibody [Unconjugated]

  • Cell proliferation-inducing gene 35 protein
  • EPC-1
  • EPC-1PIG35
  • PEDF
  • PEDFpigment epithelium-derived factor
  • pigment epithelium derived factor), member 1
  • proliferation-inducing protein 35
  • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
  • Serpin F1
  • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Background

Serpin F1 is a secreted glycoprotein that shares sequence and structural similarity with the serpin (serine proteinase inhibitor) superfamily proteins but lacks serine protease inhibitory activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Serpin F1/PEDF
Antibody [Unconjugate
AF1149
Serpin F1/PEDF Antibody [Unconjugated]
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Applications: WB, Simple Western

Publications for Serpin F1/PEDF Antibody (AF1149)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: ELISA Development, Western Blot.


Filter By Application
ELISA Development
(1)
Western Blot
(2)
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Mouse
(1)
Rat
(2)
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Showing Publications 1 - 6 of 6.
Publications using AF1149 Applications Species
C Barbé, J Salles, C Chambon, C Giraudet, P Sanchez, V Patrac, P Denis, Y Boirie, S Walrand, M Gueugneau Characterization of the Skeletal Muscle Proteome in Undernourished Old Rats International Journal of Molecular Sciences, 2022-04-26;23(9):. 2022-04-26 [PMID: 35563153] (Western Blot, Rat) Western Blot Rat
De Miguel Z, Khoury N, Betley MJ et al. Exercise plasma boosts memory and dampens brain inflammation via clusterin Nature 2021-12-16 [PMID: 34880498]
M Eslani, I Putra, X Shen, J Hamouie, N Afsharkham, S Besharat, MI Rosenblatt, R Dana, P Hematti, AR Djalilian Corneal Mesenchymal Stromal Cells Are Directly Antiangiogenic via PEDF and sFLT-1 Invest. Ophthalmol. Vis. Sci., 2017-10-01;58(12):5507-5517. 10/1/2017 [PMID: 29075761]
Shen W, Chung S, Irhimeh M, Li S, Lee S, Gillies M Systemic administration of erythropoietin inhibits retinopathy in RCS rats. PLoS ONE, 2014-08-13;9(8):e104759. 2014-08-13 [PMID: 25119659] (Western Blot, Rat) Western Blot Rat
Unterlauft JD, Eichler W, Kuhne K et al. Pigment Epithelium-Derived Factor Released by Muller Glial Cells Exerts Neuroprotective Effects on Retinal Ganglion Cells Neurochem Res 3/13/2012 [PMID: 22410737]
Streck CJ, Zhang Y, Zhou J, Ng C, Nathwani AC, Davidoff AM Adeno-associated virus vector-mediated delivery of pigment epithelium-derived factor restricts neuroblastoma angiogenesis and growth. J. Pediatr. Surg., 2005-01-01;40(1):236-43. 2005-01-01 [PMID: 15868591] (ELISA Development, Mouse) ELISA Development Mouse

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SERPINF1
Uniprot