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VEGFR1/Flt-1 Antibody [Unconjugated]

 (73 Publications)
 (2 Reviews)
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VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
VEGFR1/Flt‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Human VEGFR1/Flt‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) overnight at 4 ...read more
VEGFR1/Flt-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human VEGFR1/Flt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF321) at 3 µg/mL overnight ...read more
Exogenous VEGF165 suppresses RASSF1A expression in normal epithelial and endothelial cells. Metastatic colon cancer cells (mCRC, (A) T84 and (B) Colo 205) were stimulated with VEGF165 as indicated before and cell ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF321
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF321-SP
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VEGFR1/Flt-1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR1/Flt-1
Ser27-His687
Accession # NP_001153392
Specificity
Detects human VEGFR1/Flt-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FLT1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF321 in the following applications:
Publications
Read Publications using
AF321 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR1/Flt-1 Antibody [Unconjugated]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGFR1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR1/Flt-1 Antibody (AF321)(73)

We have publications tested in 2 confirmed species: Human, Bovine.

We have publications tested in 10 applications: Blocking, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Blocking
(1)
Flow Cytometry
(1)
Functional Assay
(2)
ICC
(5)
IHC
(8)
IHC-Fr
(2)
IHC-P
(4)
Neutralization
(12)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(14)
All Applications
Filter By Species
Human
(43)
Bovine
(2)
All Species
Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using AF321 Applications Species
Yong, Q;Dijkstra, KL;van der Keur, C;Bruijn, JA;Eikmans, M;Baelde, HJ; MIF Increases sFLT1 Expression in Early Uncomplicated Pregnancy and Preeclampsia International journal of molecular sciences 2023-06-13 [PMID: 37373198] (IHC, Human) IHC Human
X Huang, L Jia, Y Jia, X Xu, R Wang, M Wei, H Li, H Peng, Y Wei, Q He, K Wang sFlt-1-enriched exosomes induced endothelial cell dysfunction and a preeclampsia-like phenotype in mice Cytokine, 2023-04-14;166(0):156190. 2023-04-14 [PMID: 37062152] (Western Blot, Human) Western Blot Human
X Liu, Z Li, J Sun, Z Zhang, W Li Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages BMC Cancer, 2023-03-20;23(1):259. 2023-03-20 [PMID: 36941554] (Functional Assay, Human) Functional Assay Human
F Zakeri, H Latifi-Nav, ZS Soheili, M Sadeghi, SS Arab, S Samiei, ER Pirmardan, S Taghizadeh, H Ahmadieh, A Hafezi-Mog Design, construction and in vivo functional assessment of a hinge truncated sFLT01 Gene Therapy, 2022-09-16;0(0):. 2022-09-16 [PMID: 36114375] (Western Blot, Human) Western Blot Human
A Wrona, V Aleksandro, T Bereza, P Basta, A Gil, M Ulatowska-, M Mazur-Lask, K Pity?ski, K Gil Oviductal Oxygen Homeostasis in Patients with Uterine Myoma: Correlation between Hypoxia and Telocytes International Journal of Molecular Sciences, 2022-05-31;23(11):. 2022-05-31 [PMID: 35682833] (IHC, Human) IHC Human
DN Mitroi, M Tian, R Kawaguchi, WE Lowry, ST Carmichael Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia Journal of Cellular and Molecular Medicine, 2022-05-11;0(0):. 2022-05-11 [PMID: 35543222] (IHC, Human) IHC Human
J Chen, Y Wang, S Wang, X Zhao, L Zhao, Y Wang Salvianolic acid B and ferulic acid synergistically promote angiogenesis in HUVECs and zebrafish via regulating VEGF signaling Journal of ethnopharmacology, 2021-09-29;0(0):114667. 2021-09-29 [PMID: 34597652] (Western Blot, Human) Western Blot Human
V Aleksandro, A Wrona, T Bereza, K Pity?ski, K Gil Oviductal Telocytes in Patients with Uterine Myoma Biomedicines, 2021-08-20;9(8):. 2021-08-20 [PMID: 34440264] (IHC, Human) IHC Human
Shun-Yun Cheng, Yongwen Luo, Anneliese Malachi, Jihye Ko, Qin Su, Jun Xie et al. Low-Dose Recombinant Adeno-Associated Virus-Mediated Inhibition of Vascular Endothelial Growth Factor Can Treat Neovascular Pathologies Without Inducing Retinal Vasculitis Human Gene Therapy 7/1/2021 [PMID: 34182803]
Abdelrahim Alqudah, Kelly-Ann Eastwood, Djurdja Jerotic, Naomi Todd, Denise Hoch, Ross McNally et al. FKBPL and SIRT-1 Are Downregulated by Diabetes in Pregnancy Impacting on Angiogenesis and Endothelial Function Front Endocrinol (Lausanne) 6/2/2021 [PMID: 34149611]
Show All 73 Publications.

Reviews for VEGFR1/Flt-1 Antibody (AF321) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF321:
Filter by Applications
Flow
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 Flow Human 01/07/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 19757485
SpeciesHuman
  4
reviewed by:
Verified Customer
 WB Human 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21699503
SpeciesHuman

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Secondary Antibodies

 

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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 Verified Customer
01/07/2015
Application: Flow
Species: Human
read this review
 
 Verified Customer
01/07/2015
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol FLT1
Uniprot