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RP105/CD180 Antibody - BSA Free

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Western Blot: RP105/CD180 Antibody [NBP1-76706] - Human spleen tissue lysate with RP105 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: RP105/CD180 Antibody [NBP1-76706] - Human spleen tissue with RP105 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: RP105/CD180 Antibody [NBP1-76706] - Human spleen tissue with RP105 antibody at 2 ug/ml.
Immunohistochemistry: RP105/CD180 Antibody - BSA Free [NBP1-76706] - Immunohistochemistry of RP105/CD180 in human spleen tissue with RP105/CD180 antibody at 2 u/mL.
Immunohistochemistry: RP105/CD180 Antibody - BSA Free [NBP1-76706] - Immunohistochemistry of RP105/CD180 in human spleen tissue with RP105/CD180 antibody at 2 u/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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RP105/CD180 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 11 amino acid synthetic peptide from near the amino terminus of human RP105. The immunogen is located within the first 50 amino acids of RP105. Amino Acid Squence: CIEKEANKTYN
Predicted Species
Mouse (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD180
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 0.5-1 ug/ml
Application Notes
Despite its predicted molecular weight, RP105/CD180 often migrates at 95 - 105 kDa.
Control Peptide
RP105/CD180 Peptide (NBP1-76706PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RP105/CD180 Antibody - BSA Free

  • CD180 antigenMGC126233
  • CD180 molecule
  • CD180
  • Ly64
  • LY64MGC126234
  • Ly78
  • Lymphocyte antigen 64
  • lymphocyte antigen-64, radioprotective, 105kDa
  • Radioprotective 105 kDa protein
  • RP105
  • RP105lymphocyte antigen 64 (mouse) homolog, radioprotective, 105kD

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway. It has also been suggested that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes. Despite its predicted molecular weight, RP105 often migrates at 95-105 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol CD180
Uniprot