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Bcl-xL Antibody

Images

 
Western Blot: Bcl-xL Antibody [NB100-56104] - WB analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is detected. Lane 8. ...read more
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in Daoy whole cell lysate using anti-Bcl-xL antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-xL expression. The bottom image was negative for Bcl-xL expression. The ...read more
Western Blot: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-xL at ~30 kDa.
Immunohistochemistry-Paraffin: Bcl-xL Antibody [NB100-56104] - Analysis of Bcl-xL in human breast carcinoma at 1:2000. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Bv, Ma, ShSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Bcl-xL Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 46-66 (ETPSAINGNPSWHLADSPAVN) of human Bcl-xL was used as immunogen; GenBank no. CAA80661.1. The immunogen sequence is 100% conserved in Bcl-XS. The immunogen sequence is 100% in multiple species including mouse, rat, dog, cow, gerbil, and sheep.
Specificity
Bcl-XL+S
Predicted Species
Bovine (100%), Gerbil (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL2L1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000
Application Notes
IHC (frozen): Users should optimize according to model and immunodetection system used (secondary reagents)
Control
Bcl-xL Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-56104 in the following applications:

Publications
Read Publications using
NB100-56104 in the following applications:

Reactivity Notes

Mouse, Canine, Rat reactivity reported in scientific literature (PMID: 15380478).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Bcl-xL Antibody

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. NB100-56104 recognizes Bcl-xL and Bcl-xS. Human Bcl-xL is a 233 amino acid protein; GenBank no. CAA80661.1. Human Bcl-xS is a 170 amino acid protein; GenBank no. CAA80662.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-xL Antibody (NB100-56104)(3)

Review for Bcl-xL Antibody (NB100-56104) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56104:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Bcl-xL NB100-56104
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4
reviewed by:
Verified Customer
WB Human 07/15/2016
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Summary

ApplicationWestern Blot
Sample TestedDaoy whole cell lysate (25ug)
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bcl-xL Antibody (NB100-56104) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Bcl-xL Antibody (NB100-56104)

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Blogs on Bcl-xL.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Recent Reviews

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5
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4
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1
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Verified Customer
07/15/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol BCL2L1
Entrez