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Resistin Antibody

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Systemic and local HBP and resistin responses in septic patients.HBP and resistin levels in plasma collected from patients on the day of inclusion were measured with ELISA, for details see Experimental procedures. (A,B) ...read more
Subcellular localization of HBP and resistin in neutrophils.Neutrophils from healthy donors were isolated and stimulated with LPS (50 ng/ml), fMLP (5 μg/ml) or streptococcal M1-protein (1 μg/ml) for 2 h. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Resistin Antibody Summary

Immunogen
E. coli-derived recombinant human Resistin
Ser17-Pro108
Accession # Q9HD89
Specificity
Detects human Resistin in direct ELISAs and Western blots. In these formats, this antibody shows less than 2% cross-reactivity with rmResistin, rmRELM alpha and rmRELM beta .
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RETN
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1359 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Resistin Antibody

  • Adipose tissue-specific secretory factor
  • ADSF
  • ADSFMGC126609
  • C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1
  • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
  • Cysteine-rich secreted protein A12-alpha-like 2
  • Cysteine-rich secreted protein FIZZ3
  • FIZZ3
  • FIZZ3MGC126603
  • found in inflammatory zone 3
  • HXCP1
  • Resistin
  • RETN
  • RETN1
  • RSTNXCP1

Background

Human Resistin is an 11-12 kDa member of a small family of inflammation-related cysteine-rich polypeptides. It is named for its antagonistic activity towards insulin (resistance to insulin) and contains five intrachain disulfide bonds that generate a mixture of alpha -helices and beta -sheets. Secreted resistin forms disulfide-linked homodimers that likely oligomerize. Resistin may also form nondisulfide-linked heterodimers with RELM beta . One alternate splice form exists that shows an in-frame 26 amino acid (aa) deletion between amino acids 40 - 65. The significance is unclear. Mature human Resistin is 50% aa identical to human RELM beta and 56% aa identical to mouse Resistin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Resistin Antibody (AF1359)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, ICC, Mass Spectometry, Western Blot.


Filter By Application
ELISA Development
(1)
ICC
(1)
Mass Spectometry
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF1359 Applications Species
Q Lin, S Kumar, U Kariyawasa, X Yang, W Yang, JT Skinner, WD Gao, RA Johns Human Resistin Induces Cardiac Dysfunction in Pulmonary Hypertension Journal of the American Heart Association, 2023-03-16;12(6):e027621. 3/16/2023 [PMID: 36927008]
Qing Lin, Chunling Fan, John T. Skinner, Elizabeth N. Hunter, Andrew A. Macdonald, Peter B. Illei et al. RELM alpha Licenses Macrophages for Damage-Associated Molecular Pattern Activation to Instigate Pulmonary Vascular Remodeling The Journal of Immunology 2019-12-01 [PMID: 31611261]
Johanna Snäll, Anna Linnér, Julia Uhlmann, Nikolai Siemens, Heike Ibold, Marton Janos et al. Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release Scientific Reports 2016-02-18 [PMID: 26887258]
Fan C, Johns B, Su Q, Kolosova I, Johns R Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members. Histochem Cell Biol, 2012-10-18;0(0):. 2012-10-18 [PMID: 23076260] (ICC, Western Blot, Human) ICC, Western Blot Human
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis. J. Immunol. Methods, 2012-02-17;378(1):72-80. 2012-02-17 [PMID: 22366959] (ELISA Development, Human) ELISA Development Human
Tubbs KA, Kiernan UA, Niederkofler EE Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling. Anal. Chem., 2006-05-15;78(10):3271-6. 2006-05-15 [PMID: 16689526] (Mass Spectometry, Human) Mass Spectometry Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RETN
Uniprot