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RELM beta Antibody [Unconjugated]

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Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2730-SP
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RELM beta Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RELM beta
Gly21-Ala105
Accession # Q99P86
Specificity
Detects mouse RELM beta in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat RELM gamma is observed and 10% cross-reactivity with recombinant mouse (rm) Resistin and rmRELM alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RETNLB
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF2730 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RELM beta Antibody [Unconjugated]

  • C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 2
  • CCRG
  • Colon and small intestine-specific cysteine-rich protein
  • Colon carcinoma-related gene protein
  • cysteine-rich secreted A12-alpha-like protein 1
  • Cysteine-rich secreted protein A12-alpha-like 1
  • Cysteine-rich secreted protein FIZZ2
  • FIZZ1
  • FIZZ2RELM-beta
  • found in inflammatory zone 1
  • HXCP2XCP2
  • RELM beta
  • RELMb
  • RELMbeta
  • resistin like beta
  • resistin-like beta
  • RETNL2

Background

RELM beta , also known as FIZZ2 (found in inflammatory zone 2) is a 9 kDa secreted, cysteine-rich protein belonging to the RELM family. By analogy to Resistin, RELM beta is expected to exist in two distinct multimeric structures corresponding to hexamers and trimers. RELM beta is synthesized by intestinal goblet cells and is secreted into the intestinal lumen. Mouse RELM beta shares 61% and 77% amino acid (aa) sequence identity with human and rat RELM beta , respectively. It also shares 52%, 71% and 46% aa sequence identity with mouse RELM alpha , RELM gamma and Resistin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RELM beta Antibody (AF2730)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RETNLB
Uniprot