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Rad1 Antibody (4126)

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Western Blot: Rad1 Antibody (4126) [NB100-346] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Rad1 antibody [4126] (NB100-346) diluted at 1:1000. The HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
4126
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Rad1 Antibody (4126) Summary

Immunogen
Amino terminal fragment of human Rad1 purified from E. coli.
Localization
Nuclear
Specificity
This is specific for Rad 1.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RAD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publication using NB100-346.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Rad1 Antibody (4126)

  • cell cycle checkpoint protein Hrad1
  • checkpoint control protein HRAD1
  • DNA repair exonuclease rad1 homolog
  • DNA repair exonuclease REC1
  • EC 3.1.11.2
  • exonuclease homolog RAD1
  • HRAD1
  • RAD1 (S. pombe) homolog
  • RAD1 homolog (S. pombe)
  • Rad1-like DNA damage checkpoint protein
  • REC1cell cycle checkpoint protein RAD1

Background

Human Rad1 is a component of a heterotrimeric PCNA-like complex that also contains the Rad9 and Hus1 proteins. This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad1 Antibody (NB100-346)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rad1 Antibody (NB100-346) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD1
Entrez