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Pref-1/DLK1/FA1 Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Unconjugated
AF1144
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1144
Carrier Free
AF1144
Carrier Free
AF1144-SP
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Pref-1/DLK1/FA1 Antibody [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform
Ala24-Pro297 with Arg248Pro and Lys295Ser substitutions
Accession # AAA75364
Specificity
Detects human Pref‑1/DLK‑1/FA1 in Western blots. In this format, approximately 25% cross-reactivity with recombinant mouse Pref‑1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DLK1
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody [Biotin]

  • delta-like 1 homolog (Drosophila)
  • DLK
  • DLK1
  • DLK-1
  • FA1
  • FA1fetal antigen 1
  • pG2
  • pG2delta-like homolog (Drosophila)
  • preadipocyte factor 1
  • Pref1
  • Pref-1
  • protein delta homolog 1
  • secredeltin
  • ZOG

Background

Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa #207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pref-1/DLK1/FA1
Antibody [Biotin]
BAF1144
Pref-1/DLK1/FA1 Antibody [Biotin]
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol DLK1
Uniprot