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MAP3K13 Antibody

Images

 
Western Blot: MAP3K13 Antibody [NBP1-81272] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: MAP3K13 Antibody [NBP1-81272] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human placenta shows strong membranous positivity in trphoblastic cells.
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human kidney shows strong membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: MAP3K13 Antibody [NBP1-81272] - Staining of human prostate shows strong membranous positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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MAP3K13 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KKYPGTYKRHPVRPIIHPNAMEKLMKRKGVPHKSGMQTKRPDLLRSEGIPTTEVAPTASPLSGSPKMSTSSSKSRYRSKPRHRRGNSRGSHSDFAAILKNQPAQENSPHPTYLHQAQSQYPSLHHHNSLQQQYQQP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP3K13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Simple Western
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. Simple Western reported by internal validation: Tested in mouse retinal ganglion cells; separated by size.
Control Peptide
MAP3K13 Protein (NBP1-81272PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (83%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAP3K13 Antibody

  • EC 2.7.11
  • EC 2.7.11.25
  • Leucine zipper-bearing kinase
  • LZK
  • LZKMGC133196
  • MAP3K13
  • MEKK13
  • mitogen-activated protein kinase kinase kinase 13
  • Mixed lineage kinase
  • MLK

Background

MAP3K13 is encoded by this gene is a member of serine/threonine protein kinase family. This kinase contains a dual leucine-zipper motif, and has been shown to form dimers/oligomers through its leucine-zipper motif. This kinase can phosphorylate and activate MAPK8/JNK, MAP2K7/MKK7, which suggests a role in the JNK signaling pathway. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAP3K13