![Western Blot: MAP3K12 Antibody [NBP2-17218] - A. 20 ug mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/MAP3K12-Antibody-Western-Blot-NBP2-17218-img0015.jpg "Western Blot: MAP3K12 Antibody [NBP2-17218] - A. 20 ug mouse embryonic stem cell whole cell lysate/extract 7.5 % SDS-PAGEDLK/MAP3K12 antibody dilution: 1:1000")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse MAP3K12. The exact sequence is proprietary. |
| Localization | Cytoplasm; Membrane; Peripheral membrane protein |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAP3K12 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western internal validation: Mouse Retinal Ganglion Cells as sample; separated by size; detected by Chemiluminescence. |
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| Theoretical MW | 96 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MAP3K12 Antibody (NBP2-17218)Find related products by research area.
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![Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218] - Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/MAP3K12-Antibody-Immunohistochemistry-Frozen-NBP2-17218-img0014.jpg "Immunohistochemistry-Frozen: MAP3K12 Antibody [NBP2-17218] - Frozen sectioned E13.5 Rat brain. Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: MAP3K12 Antibody [NBP2-17218] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/MAP3K12-Antibody-Western-Blot-NBP2-17218-img0007.jpg "Western Blot: MAP3K12 Antibody [NBP2-17218] - Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:1000.")
![Western Blot: MAP3K12 Antibody [NBP2-17218] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody iluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAP3K12-Antibody-Western-Blot-NBP2-17218-img0010.jpg "Western Blot: MAP3K12 Antibody [NBP2-17218] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody iluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/MAP3K12-Antibody-Immunohistochemistry-Paraffin-NBP2-17218-img0005.jpg "Immunohistochemistry-Paraffin: MAP3K12 Antibody [NBP2-17218] - Analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.")
![Western Blot: MAP3K12 Antibody [NBP2-17218] -Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp2-17218_rabbit-polyclonal-map3k12-antibody-western-blot-1462023112328..jpg "Western Blot: MAP3K12 Antibody [NBP2-17218] -Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody diluted at 1:1000.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")