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Pref-1/DLK1/FA1 Antibody [Unconjugated]

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 (1 Review)
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Western blot shows lysates of human adrenal gland tissue and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1144) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human adrenal gland tissue and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Pref-1/DLK1/FA1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1144) at 10 µg/mL for 3 ...read more
Dlk1 inhibits myoblast differentiation in vitro.A: C2C12 cells were constructed to constitutively express full-length Dlk1 under control by the CMV promotor, DLK1-C2C12. Cell morphology and myogenic differentiation ...read more
Dlk1 inhibits myoblast differentiation in vitro.A: C2C12 cells were constructed to constitutively express full-length Dlk1 under control by the CMV promotor, DLK1-C2C12. Cell morphology and myogenic differentiation ...read more
Dlk1 inhibits myoblast differentiation in vitro.A: C2C12 cells were constructed to constitutively express full-length Dlk1 under control by the CMV promotor, DLK1-C2C12. Cell morphology and myogenic differentiation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1144
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Catalog# & Formulation Size Price
Carrier Free
AF1144-SP
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Pref-1/DLK1/FA1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1 long isoform
Ala24-Pro297 with Arg248Pro and Lys295Ser substitutions
Accession # AAA75364
Specificity
Detects human Pref-1/DLK-1/FA1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DLK1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF1144 in the following applications:
Publications
Read Publications using
AF1144 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody [Unconjugated]

  • delta-like 1 homolog (Drosophila)
  • DLK
  • DLK1
  • DLK-1
  • FA1
  • FA1fetal antigen 1
  • pG2
  • pG2delta-like homolog (Drosophila)
  • preadipocyte factor 1
  • Pref1
  • Pref-1
  • protein delta homolog 1
  • secredeltin
  • ZOG

Background

Pref-1 (Preadipocyte factor 1; also DLK-1 and FA1) is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25‑31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1‑52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa # 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.            

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pref-1/DLK1/FA1
Antibody [Unconjugate
AF1144
Pref-1/DLK1/FA1 Antibody [Unconjugated]
Species: Hu
Applications: WB, ICC/IF

Publications for Pref-1/DLK1/FA1 Antibody (AF1144)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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(1)
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Review for Pref-1/DLK1/FA1 Antibody (AF1144) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1144:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Pref-1/DLK1/FA1 AF1144
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 05/25/2018
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Summary

ApplicationWestern Blot
Sample TestedNIH3T3 DLK1 overexpress whole cell lysate
SpeciesHuman
LotGQC0316021

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Secondary Antibodies

 

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Recent Reviews

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Western Blot Pref-1/DLK1/FA1 AF1144
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 Verified Customer
05/25/2018
Application: WB
Species: Human
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Bioinformatics

Gene Symbol DLK1
Uniprot