Home » Pref-1/DLK1/FA1 » Pref-1/DLK1/FA1 Antibodies » Pref-1/DLK1/FA1 Antibody (1168B) [Unconjugated]
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Pref-1/DLK1/FA1 Antibody (1168B) [Unconjugated]

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Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog ...read more
3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634, filled histogram) or isotype control antibody (Catalog # AB-108-C, ...read more
Pref‑1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic liver tissue (13 d.p.c.) using Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) at 1 µg/mL for 1 ...read more
Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Pref‑1/DLK1/FA1 at approximately 63 kDa (as indicated) using 1 μg/mL of Rabbit ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC
Clone
1168B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB8634U-100UG
Alexa Fluor 405
FAB8634V-100UG
Alexa Fluor 488
FAB8634G-025
Alexa Fluor 488
FAB8634G-100
Alexa Fluor 532
FAB8634X
Alexa Fluor 594
FAB8634T-100UG
Alexa Fluor 647
FAB8634R-100UG
Alexa Fluor 700
FAB8634N-100UG
Alexa Fluor 750
FAB8634S-100UG
Allophycocyanin
FAB8634A-025
Allophycocyanin
FAB8634A-100
Allophycocyanin/Cy7
FAB8634APCCY7
Biotin
FAB8634B
CoraFluor 1
FAB8634CL1
DyLight 350
FAB8634D
DyLight 405
FAB8634E
DyLight 488
FAB8634K
DyLight 550
FAB8634L
DyLight 594
FAB8634M
DyLight 650
FAB8634W
DyLight 680
FAB8634Y
DyLight 755
FAB8634Z
FITC
FAB8634F
HRP
FAB8634H
Janelia Fluor 525
FAB8634JF525
Janelia Fluor 549
FAB8634I
Janelia Fluor 646
FAB8634J
Janelia Fluor 669
FAB8634JF669
PE/Atto594
FAB8634PEATT594
PE/Cy5.5
FAB8634PECY55
PE/Cy7
FAB8634PECY7
PerCP
FAB8634C
Phycoerythrin
FAB8634P-100
Phycoerythrin
FAB8634P-025
mFluor Violet 450 SE
FAB8634MFV450
mFluor Violet 500 SE
FAB8634MFV500
mFluor Violet 610 SE
FAB8634MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB8634P-025
Carrier Free
MAB8634-SP
Carrier Free
FAB8634A-025
Carrier Free
FAB8634G-025
Carrier Free
FAB8634A-100
Carrier Free
FAB8634P-100
Carrier Free
FAB8634G-100
Carrier Free
FAB8634V-100UG
Carrier Free
FAB8634U-100UG
Carrier Free
FAB8634T-100UG
Carrier Free
FAB8634S-100UG
Carrier Free
FAB8634R-100UG
Carrier Free
FAB8634N-100UG
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Pref-1/DLK1/FA1 Antibody (1168B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163
Specificity
Detects mouse Pref-1 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
DLK1
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-25 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
MAB8634 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody (1168B) [Unconjugated]

  • delta-like 1 homolog (Drosophila)
  • DLK
  • DLK1
  • DLK-1
  • FA1
  • FA1fetal antigen 1
  • pG2
  • pG2delta-like homolog (Drosophila)
  • preadipocyte factor 1
  • Pref1
  • Pref-1
  • protein delta homolog 1
  • secredeltin
  • ZOG

Background

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385).  It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pref-1/DLK1/FA1
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MAB8634
Pref-1/DLK1/FA1 Antibody (1168B) [Unconjugated]
Species: Mu
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Publications for Pref-1/DLK1/FA1 Antibody (MAB8634)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC.


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Bioinformatics

Gene Symbol DLK1
Uniprot