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POLR2D Antibody - BSA Free

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Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of 200ug extracts of HepG2 cells using POLR2D at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: POLR2D Antibody [NBP2-93656] - Analysis of A549 cells using POLR2D .
Western Blot: POLR2D Antibody [NBP2-93656] - Analysis of extracts of various cell lines, using POLR2D at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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POLR2D Antibody - BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1). MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POLR2D
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:100
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for POLR2D Antibody - BSA Free

  • DNA-directed RNA polymerase II 16 kDa polypeptide
  • DNA-directed RNA polymerase II subunit D
  • DNA-directed RNA polymerase II subunit RPB4
  • HSRBP4
  • HSRPB4
  • polymerase (RNA) II (DNA directed) polypeptide D
  • RBP4
  • RNA polymerase II 16 kDa subunit
  • RNA polymerase II subunit B4
  • RNA polymerase II subunit hsRBP4
  • RPB16

Background

The POLR2D gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for POLR2D Antibody (NBP2-93656) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POLR2D