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PIWIL1/HIWI Antibody - BSA Free

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Western Blot: PIWIL1/HIWI Antibody [NBP1-76489] - Analysis of PIWI-L1 in human testis tissue lysate with PIWI-L2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: PIWIL1/HIWI Antibody - BSA Free [NBP1-76489] - PIWIL1/HIWI Antibody [NBP1-76489] - Rat liver tissue with PIWI-L1 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody [NBP1-76489] - Rat liver tissue with PIWI-L1 antibody at 10 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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PIWIL1/HIWI Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1. The immunogen is located within amino acids 60 - 110 of PIWI-L1. Amino Acid Squence: ELSLAERGGRRRDFHDLG
Predicted Species
Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIWIL1
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PIWIL1/HIWI Peptide (NBP1-76489PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PIWIL1/HIWI Antibody - BSA Free

  • HIWI
  • HIWIpiwi homolog
  • MIWI
  • piwi (Drosophila)-like 1
  • PIWI
  • PIWIL1
  • piwi-like 1 (Drosophila)
  • piwi-like protein 1

Background

PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PIWIL1
Uniprot