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PIWIL3 Antibody - BSA Free

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Western Blot: PIWIL3 Antibody [NBP1-76490] - 3T3 cell lysate with PIWI-L3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: PIWIL3 Antibody [NBP1-76490] - Immunofluorescence of PIWI-L3 in 3T3 cells with PIWI-L3 antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: PIWIL3 Antibody - BSA Free [NBP1-76490] - Immunofluorescence of PIWIL3 in 3T3 cells with PIWIL3 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: PIWIL3 Antibody - BSA Free [NBP1-76490] - Immunofluorescence of PIWIL3 in 3T3 cells with PIWIL3 antibody at 20 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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PIWIL3 Antibody - BSA Free Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L3. The immunogen is located within amino acids 130 - 180 of PIWI-L3. Amino Acid Squence: YKYNVDYKPDIEDGNLRT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIWIL3
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
PIWIL3 Peptide (NBP1-76490PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PIWIL3 Antibody - BSA Free

  • HIWI3
  • piwi-like 3 (Drosophila)
  • piwi-like protein 3

Background

PIWI-L3 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. Recent studies have shown that overexpression of PIWI-L3 (as well as other PIWI-like proteins) are potential biomarkers for astrocytic glioma, meningioma, and other cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PIWIL3
Uniprot