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PIWIL4 Antibody (10G9B11) - BSA Free

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Western Blot: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Western blot analysis using PIWIL4 mouse mAb against PC-3 (1) and PANC-1 (2) cell lysate.
Immunohistochemistry: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 mouse mAb with DAB staining.
Flow Cytometry: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Flow cytometric analysis of HeLa cells using PIWIL4 mouse mAb (green) and negative control (purple).
Western Blot: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Western blot analysis using PIWIL4 mAb against human PIWIL4 recombinant protein. (Expected MW is 40.8 kDa)
Western Blot: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Western blot analysis using PIWIL4 mAb against HEK293 (1) and PIWIL4 (AA: 304-434)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 mouse mAb with DAB staining.
ELISA: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
10G9B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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PIWIL4 Antibody (10G9B11) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PIWIL4 (AA: 304-434) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PIWIL4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
96.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PIWIL4 Antibody (10G9B11) - BSA Free

  • DKFZp686P01248
  • FLJ36156
  • HIWI2
  • HIWI2MIWI2
  • MIWI2
  • PIWI
  • PIWIL4
  • piwi-like 4 (Drosophila)
  • piwi-like protein 4

Background

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PIWIL4