![Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Drosha-Antibody-Western-Blot-NBP1-03349-img0006.jpg "Western Blot: Drosha Antibody [NBP1-03349] - Detection of Human Drosha by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1324 and 1374 of human double-stranded RNA-specific endoribonuclease using the numbering given in entry NP_037367.3 (GeneID 29102). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DROSHA |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Centrifuge tube to remove product from lid. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IP | Human | 07/06/2015 |
Summary
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reviewed by:
Verified Customer |
WB | Human | 07/06/2015 |
Summary
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reviewed by:
rajesha rupaimoole |
WB | Human | 10/06/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Drosha Antibody (NBP1-03349)Find related products by research area.
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| Verified Customer 07/06/2015 |
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| Application: | IP | |
| Species: | Human |
| Verified Customer 07/06/2015 |
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| Application: | WB | |
| Species: | Human |
| rajesha rupaimoole 10/06/2014 |
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| Application: | WB | |
| Species: | Human |
![Immunoprecipitation: Drosha Antibody [NBP1-03349] - Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by another rabbit anti-Drosha antibody. For blotting immunoprecipitated Drosha, NBP1-03349 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Drosha-Antibody-Immunoprecipitation-NBP1-03349-img0007.jpg "Immunoprecipitation: Drosha Antibody [NBP1-03349] - Detection of human Drosha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Drosha antibody NBP1-03349 used for IP at 6 ug per reaction. Drosha was also immunoprecipitated by another rabbit anti-Drosha antibody. For blotting immunoprecipitated Drosha, NBP1-03349 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Flow Cytometry CD1b Antibody (737249) [Unconjugated]](https://images.novusbio.com/images2/CD1b_MAB7446_Flow_Cytometry_12185.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
