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FHL5 Antibody

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Western Blot: FHL5 Antibody [NBP1-32660] - Sample (30 ug of whole cell lysate) A: 293T B: Raji 12 % SDS PAGE, antibody diluted at 1:1000.
Western Blot: FHL5 Antibody [NBP1-32660] - Sample (30 ug of whole cell lysate) A: 293T B: Raji 12 % SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FHL5 Antibody [NBP1-32660] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: FHL5 Antibody [NBP1-32660] - SW480 xenograft , using FHL5 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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FHL5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FHL5. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Chimpanzee (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FHL5
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-32660 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23127979).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for FHL5 Antibody

  • activator of cAMP-responsive element modulator (CREM) in testis
  • Activator of cAMP-responsive element modulator in testis
  • Activator of CREM in testis
  • ACTKIAA0776
  • dJ393D12.2
  • FHL-5
  • FLJ33049
  • four and a half LIM domains 5,1700027G07Rik
  • four and a half LIM domains protein 5
  • LIM protein ACT
  • RP3-393D12.2

Background

The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator (CREM). It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FHL5 Antibody (NBP1-32660)(1)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


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(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
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FAQs for FHL5 Antibody (NBP1-32660) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FHL5