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NHLH1 Antibody

Images

 
Western Blot: NHLH1 Antibody [NBP2-13655] - Analysis in control (vector only transfected HEK293T lysate) and NHLH1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NHLH1 Antibody [NBP2-13655] - Staining of human cell line AF22 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NHLH1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: MMLNSDTMELDLPPTHSETESGFSDCGGGAGPDGAGPGGPGGGQARGPEPGEPGRKDLQHLSREE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NHLH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NHLH1 Protein (NBP2-13655PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NHLH1 Antibody

  • BHLHA35
  • bHLHa35HEN-1
  • helix-loop-helix protein 1
  • HEN1NSCL-1
  • nescient helix loop helix 1Class A basic helix-loop-helix protein 35
  • NSCL
  • NSCL1

Background

The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NHLH1 Antibody (NBP2-13655) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NHLH1