Name: VASA Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2030
Price: 489 USD

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2030

Immunogen	E. coli-derived recombinant human VASA Met1-Tyr145 Accession # Q9NQI0
Specificity	Detects human VASA in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	DDX4
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

VASA Antibody [Unconjugated]

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Publications for VASA Antibody (AF2030)(57)

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Bioinformatics

Publications using AF2030	Applications	Species
Baskin, LS;Cao, M;Li, Y;Baker, L;Cooper, CS;Cunha, GR; Ovotesticular cords and ovotesticular follicles: New histologic markers for human ovotesticular syndrome Journal of pediatric urology 2024-01-06 [PMID: 38218629] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Penny A. F. Whiley, Michael C. M. Luu, Liza O'Donnell, David J. Handelsman, Kate L. Loveland Testis exposure to unopposed/elevated activin A in utero affects somatic and germ cells and alters steroid levels mimicking phthalate exposure Front Endocrinol (Lausanne) 2023-09-01 [PMID: 37727456]
M. Bashawat, B.C. Braun, K. Müller, B.P. Hermann Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development – A model for wild felids Theriogenology Wild 2023-07-18 [PMID: 37461433]
Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay Cell Reports Methods 2023-06-26 [PMID: 37426764]
Zhang, X;Liu, Y;Sosa, F;Gunewardena, S;Crawford, PA;Zielen, AC;Orwig, KE;Wang, N; Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cells Cell reports 2023-07-04 [PMID: 37405912] (ICC/IF, Western Blot, IHC, Mouse)	ICC/IF, Western Blot, IHC	Mouse
AS Bailey, MT Fuller YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiation bioRxiv : the preprint server for biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36747642] (IHC, Mouse)	IHC	Mouse
Yasunari Seita, Keren Cheng, John R McCarrey, Nomesh Yadu, Ian H Cheeseman, Alec Bagwell et al. Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cells eLife 2023-01-31 [PMID: 36719274]
Zhao S, Huang C, Yang Y et al. DNA repair protein FANCD2 has both ubiquitination-dependent and ubiquitination-independent functions during germ cell development Journal of Biological Chemistry 2023-03-01 [PMID: 36642183]
T Lehtiniemi, M Bourgery, L Ma, A Ahmedani, M Mäkelä, J Asteljoki, O Olotu, S Laasanen, FP Zhang, K Tan, JN Chousal, D Burow, S Koskinen, A Laiho, LL Elo, F Chalmel, MF Wilkinson, N Kotaja SMG6 localizes to the chromatoid body and shapes the male germ cell transcriptome to drive spermatogenesis Nucleic Acids Research, 2022-11-11;0(0):. 2022-11-11 [PMID: 36259644] (IHC, Human)	IHC	Human
Sarah C. Moody, Penny A. F. Whiley, Patrick S. Western, Kate L. Loveland The Impact of Activin A on Fetal Gonocytes: Chronic Versus Acute Exposure Outcomes Front Endocrinol (Lausanne) 2022-05-31 [PMID: 35721752]
Xin Mi, Wenlin Jiao, Yajuan Yang, Yingying Qin, Zi-Jiang Chen, Shidou Zhao HGF Secreted by Mesenchymal Stromal Cells Promotes Primordial Follicle Activation by Increasing the Activity of the PI3K-AKT Signaling Pathway Stem Cell Reviews and Reports 2022-06-01 [PMID: 35089464]
SP Windley, C Mayère, AE McGovern, NL Harvey, S Nef, Q Schwarz, S Kumar, D Wilhelm Loss of NEDD4 causes complete XY gonadal sex reversal in mice Cell Death & Disease, 2022-01-24;13(1):75. 2022-01-24 [PMID: 35075134] (IHC, Mouse)	IHC	Mouse
Yoji Kojima, Chika Yamashiro, Yusuke Murase, Yukihiro Yabuta, Ikuhiro Okamoto, Chizuru Iwatani et al. GATA transcription factors, SOX17 and TFAP2C, drive the human germ-cell specification program Life Science Alliance 2021-05-01 [PMID: 33608411]
J Guo, E Sosa, T Chitiashvi, X Nie, EJ Rojas, E Oliver, DonorConne, K Plath, JM Hotaling, JB Stukenborg, AT Clark, BR Cairns Single-cell analysis of the developing human testis reveals somatic niche cell specification and fetal germline stem cell establishment Cell Stem Cell, 2021-01-15;0(0):. 2021-01-15 [PMID: 33453151] (IHC, Human)	IHC	Human
Shetty G, Mitchell JM, Meyer JM et al. Restoration of functional sperm production in irradiated pubertal rhesus monkeys by spermatogonial stem cell transplantation Andrology 4/29/2020 [PMID: 32351003]
S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice PLoS ONE, 2020-01-07;15(1):e0227411. 2020-01-07 [PMID: 31910233] (IF, Mouse)	IF	Mouse
Lihua Dong, Murat Gul, Simone Hildorf, Susanne Elisabeth Pors, Stine Gry Kristensen, Eva R. Hoffmann et al. Xeno-Free Propagation of Spermatogonial Stem Cells from Infant Boys International Journal of Molecular Sciences 2019-10-29 [PMID: 31671863]
Lihua Dong, Stine Gry Kristensen, Simone Hildorf, Murat Gul, Erik Clasen-Linde, Jens Fedder et al. Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys Frontiers in Physiology 2019-09-19 [PMID: 31607938]
Sandra Laurentino, Laura Heckmann, Sara Di Persio, Xiaolin Li, Gerd Meyer zu Hörste, Joachim Wistuba et al. High-resolution analysis of germ cells from men with sex chromosomal aneuploidies reveals normal transcriptome but impaired imprinting Clinical Epigenetics 2019-12-01 [PMID: 31462300]
Mina L Kojima, Dirk G de Rooij, David C Page Amplification of a broad transcriptional program by a common factor triggers the meiotic cell cycle in mice eLife 2019-02-27 [PMID: 30810530]
GUNAPALA Shetty, ZHUANG Wu, TRUONG NGUYEN ANH LAM, THIEN TRONG Phan, KYLE E Orwig, MARVIN L Meistrich Effect of hormone modulations on donor‐derived spermatogenesis or colonization after syngeneic and xenotransplantation in mice Andrology 2019-03-01 [PMID: 30471208]
BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids Cell Rep, 2018-11-06;25(6):1650-1667.e8. 2018-11-06 [PMID: 30404016] (IHC-Fr, Human)	IHC-Fr	Human
JM Stringer, SC Forster, Z Qu, L Prokopuk, MK O'Bryan, DK Gardner, SJ White, D Adelson, PS Western Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance BMC Biol., 2018-09-20;16(1):104. 2018-09-20 [PMID: 30236109] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Nicholas Serra, Ellen K Velte, Bryan A Niedenberger, Oleksander Kirsanov, Christopher B Geyer The mTORC1 component RPTOR is required for maintenance of the foundational spermatogonial stem cell pool in mice† Biology of Reproduction 2019-02-01 [PMID: 30202948]
Á Vértesy, W Arindrarto, MS Roost, B Reinius, V Torrens-Ju, M Bialecka, I Moustakas, Y Ariyurek, E Kuijk, H Mei, R Sandberg, A van Oudena, SM Chuva de S Parental haplotype-specific single-cell transcriptomics reveal incomplete epigenetic reprogramming in human female germ cells Nat Commun, 2018-05-14;9(1):1873. 2018-05-14 [PMID: 29760424] (IHC, Human)	IHC	Human
A Bothun, Y Gao, Y Takai, O Ishihara, H Seki, B Karger, J Tilly, DC Woods Quantitative proteomic profiling of the human ovary from early to mid-gestation reveals protein expression dynamics of oogenesis and folliculogenesis Stem Cells Dev., 2018-05-29;0(0):. 2018-05-29 [PMID: 29631484] (ICC, Human)	ICC	Human
JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Stem Cell Res, 2018-01-04;27(0):46-56. 2018-01-04 [PMID: 29324254] (IHC-P, Human)	IHC-P	Human
Alexis S Bailey, Pedro J Batista, Rebecca S Gold, Y Grace Chen, Dirk G de Rooij, Howard Y Chang et al. The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline eLife 2017-10-31 [PMID: 29087293]
N Wang, C Satirapod, Y Ohguchi, ES Park, DC Woods, JL Tilly Genetic studies in mice directly link oocytes produced during adulthood to ovarian function and natural fertility Sci Rep, 2017-08-30;7(1):10011. 2017-08-30 [PMID: 28855574] (IHC, Mouse)	IHC	Mouse
AT Clark, S Gkountela, D Chen, W Liu, E Sosa, M Sukhwani, JD Hennebold, KE Orwig Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23 Stem Cell Reports, 2017-06-01;0(0):. 2017-06-01 [PMID: 28579394] (ICC, IHC, Primate - Macaca mulatta (Rhesus Macaque))	ICC, IHC	Primate - Macaca mulatta (Rhesus Macaque)
C Eguizabal Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming Stem Cells, 2016-06-30;0(0):. 2016-06-30 [PMID: 27300161] (IHC-P, Human)	IHC-P	Human
Heeren AM, He N, de Souza AF et al. On the development of extragonadal and gonadal human germ cells. Biol Open. 2/1/2016 [PMID: 26834021]
Bentolhoda Fereydouni, Gabriela Salinas-Riester, Michael Heistermann, Ralf Dressel, Lucia Lewerich, Charis Drummer et al. Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary Stem Cells International 2016-01-01 [PMID: 26664406]
Jonathan T. Busada, Bryan A. Niedenberger, Ellen K. Velte, Brett D. Keiper, Christopher B. Geyer Mammalian target of rapamycin complex 1 (mTORC1) Is required for mouse spermatogonial differentiation in vivo Developmental Biology 2015-11-01 [PMID: 26254600]
Hu , Yueh-Chi, Nicholls , Peter K, Soh , Y Q Shir, Daniele , Joseph R, Junker , Jan Phil, van Oudenaarden , Alexande, Page , David C Licensing of primordial germ cells for gametogenesis depends on genital ridge signaling. PLoS Genet, 2015-03-04;11(3):e1005019. 2015-03-04 [PMID: 25739037] (IHC-P, Mouse)	IHC-P	Mouse
Bryan A. Niedenberger, Jonathan T. Busada, Christopher B. Geyer Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis REPRODUCTION 2015-04-01 [PMID: 25737569]
Heeren A, van Iperen L, Klootwijk D, de Melo Bernardo A, Roost M, Gomes Fernandes M, Louwe L, Hilders C, Helmerhorst F, van der Westerlaken L, Chuva de Sousa Lopes S Development of the follicular basement membrane during human gametogenesis and early folliculogenesis. BMC Dev Biol, 2015-01-21;15(0):4. 2015-01-21 [PMID: 25605128] (IHC-P, Human)	IHC-P	Human
Ziwei Li, Juehua Yu, Linzi Hosohama, Kevin Nee, Sofia Gkountela, Sonal Chaudhari et al. The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice The EMBO Journal 2015-03-12 [PMID: 25519955]
William A. Pastor, Hume Stroud, Kevin Nee, Wanlu Liu, Dubravka Pezic, Sergei Manakov et al. MORC1 represses transposable elements in the mouse male germline Nature Communications 2014-12-12 [PMID: 25503965]
Dominguez , Antonia, Chiang , H Rosari, Sukhwani , Meena, Orwig , Kyle E, Reijo Pera , Renee A Human germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Sci Rep, 2014-09-22;4(0):6432. 2014-09-22 [PMID: 25242416] (IHC-P, Mouse)	IHC-P	Mouse
Eran Altman, Pamela Yango, Radwa Moustafa, James F. Smith, Peter C. Klatsky, Nam D. Tran Characterization of human spermatogonial stem cell markers in fetal, pediatric, and adult testicular tissues REPRODUCTION 2014-10-01 [PMID: 25030892]
B Fereydouni, C Drummer, N Aeckerle, S Schlatt, R Behr The neonatal marmoset monkey ovary is very primitive exhibiting many oogonia REPRODUCTION 2014-08-01 [PMID: 24840529]
Lim J, Shim M, Lee J, Lee D Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells. PLoS ONE, 2014-04-01;9(4):e90454. 2014-04-01 [PMID: 24690677] (ICC, Human)	ICC	Human
Yueh-Chiang Hu, Dirk G. de Rooij, David C. Page Tumor suppressor gene Rb is required for self-renewal of spermatogonial stem cells in mice Proceedings of the National Academy of Sciences 2013-07-30 [PMID: 23858447]
Duggal G, Heindryckx B, Warrier S, O'Leary T, Van der Jeught M, Lierman S, Vossaert L, Deroo T, Deforce D, Chuva de Sousa Lopes S, De Sutter P Influence of activin A supplementation during human embryonic stem cell derivation on germ cell differentiation potential. Stem Cells Dev, 2013-08-14;22(23):3141-55. 2013-08-14 [PMID: 23829223] (ICC, Human)	ICC	Human
Bhartiya , Deepa, Sriraman , Kalpana, Gunjal , Pranesh, Modak , Harshada Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries? J Ovarian Res, 2012-11-07;5(1):32. 2012-11-07 [PMID: 23134576] (IHC, Mouse)	IHC	Mouse
Yosef Buganim, Elena Itskovich, Yueh-Chiang Hu, Albert W. Cheng, Kibibi Ganz, Sovan Sarkar et al. Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like Cells by Defined Factors Cell Stem Cell 2012-09-07 [PMID: 22958931]
Sonya M. Schuh-Huerta, Nicholas A. Johnson, Mitchell P. Rosen, Barbara Sternfeld, Marcelle I. Cedars, Renee A. Reijo Pera Genetic markers of ovarian follicle number and menopause in women of multiple ethnicities Human Genetics 2012-11-01 [PMID: 22696150]
N. Aeckerle, K. Eildermann, C. Drummer, J. Ehmcke, S. Schweyer, A. Lerchl et al. The pluripotency factor LIN28 in monkey and human testes: a marker for spermatogonial stem cells? MHR: Basic science of reproductive medicine 2012-10-01 [PMID: 22689537]
White YA, Woods DC, Takai Y, Ishihara O, Seki H, Tilly JL. Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women. Nat Med;18(3):413-21. 2/26/2012 [PMID: 22366948]
Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC Complete meiosis from human induced pluripotent stem cells. Stem Cells, 2011-08-01;29(8):1186-95. 2011-08-01 [PMID: 21681858] (ICC, Human)	ICC	Human
Geens M, Sermon KD Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells. J. Assist. Reprod. Genet., 2011-02-12;0(0):. 2011-02-12 [PMID: 21318592] (ICC, IHC-P, Human)	ICC, IHC-P	Human
West FD, Mumaw JL, Gallegos-Cardenas A, Young A, Stice SL Human haploid cells differentiated from meiotic competent clonal germ cell lines that originated from embryonic stem cells. Stem Cells Dev., 2010-12-02;20(6):1079-88. 2010-12-02 [PMID: 20929355] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T Generation of pluripotent stem cells from adult human testis. Nature, 2008-10-08;456(7220):344-9. 2008-10-08 [PMID: 18849962] (ICC, Human)	ICC	Human
Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells. Stem Cells, 2009-01-01;0(0):. 2009-01-01 [PMID: 18845765] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Tilgner K, Atkinson SP, Golebiewska A, Stojkovic M, Lako M, Armstrong L Isolation of primordial germ cells from differentiating human embryonic stem cells. Stem Cells, 2008-09-18;26(12):3075-85. 2008-09-18 [PMID: 18802037] (ICC, Human)	ICC	Human
West FD, Machacek DW, Boyd NL, Pandiyan K, Robbins KR, Stice SL Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling. Stem Cells, 2008-08-21;26(11):2768-76. 2008-08-21 [PMID: 18719225] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
Show All 57 Publications. Collapse Publications.

Catalog# & Formulation	Size	Price
Carrier Free AF2030-SP

Gene Symbol	DDX4
Uniprot	Q9NQI0()