![Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Western-Blot-NB110-40765-img0003.jpg "Western Blot: Perilipin-3/TIP47 Antibody [NB110-40765] - Detection of TIP47 in 3T3 L1 lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | A synthetic peptide made to a region within the N-terminus (within residues 1-100) of the mouse TIP47 protein. [Swiss-Prot# Q9DBG5] |
| Localization | Cytoplasm. Endosome. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLIN3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This TIP47 antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In Western Blot analysis, a band is seen approx. 47 kDa (isoform B, containing the first 184 residues that isoform A is lacking). In ICC/IF, endosomal staining was observed in U2OS cells. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | Tris-Glycine and 0.15M NaCl |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publications using NB110-40765 | Applications | Species |
|---|---|---|
| Zhao Y, Albrecht E, Li Z et al. Distinct Roles of Perilipins in the Intramuscular Deposition of Lipids in Glutamine-Supplemented, Low-, and Normal-Birth-Weight Piglets Frontiers in Veterinary Science 2021-06-21 [PMID: 34235195] (Immunohistochemistry) | Immunohistochemistry | |
| Asimakopoulou A, Vucur M, Luedde T et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma Cancers (Basel) 2019-03-19 [PMID: 30893876] (WB, Human, Mouse) | WB | Human, Mouse |
| Imanishi Y et al. Retinyl ester homeostasis in the adipose differentiation-related protein-deficient retina. J Biol Chem 283(36):25091-102. 2008-09-05 [PMID: 18606814] (WB, Mouse) | WB | Mouse |
| Dahl N Development of skeletal muscle and adipose tissues in neonatal dairy calves upon a maternal supplementation with essential fatty acids and conjugated linoleic acids Thesis 2022-01-01 |
Secondary Antibodies |
Isotype Controls |
Research Areas for Perilipin-3/TIP47 Antibody (NB110-40765)Find related products by research area.
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| Gene Symbol | PLIN3 |
![Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40765-img0004.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-3/TIP47 Antibody [NB110-40765] - TIP47 antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
![Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Perilipin-3-TIP47-Antibody-Simple-Western-NB110-40765-img0005.jpg "Simple Western: Perilipin-3/TIP47 Antibody [NB110-40765] - Simple Western lane view shows a specific band for TIP47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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