![Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue, breast, colon and liver using Anti-Perilipin-4 antibody NBP2-13776 (A) shows similar protein distribution across tissues to independent antibody NBP2-38731 (B).](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-13776-img0023.jpg "Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue, breast, colon and liver using Anti-Perilipin-4 antibody NBP2-13776 (A) shows similar protein distribution across tissues to independent antibody NBP2-38731 (B).")
| Reactivity | Hu, Mu, PoSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a recombinant protein corresponding to the amino acids: GVDTSKTVLTGTKDTVCSGVTGAMNVAKGTIQTGVDTTKTVLTGTKNTVCSGVTGAVNLAKEAIQGGLDTTKSMVMGTKDTMSTGLTGAANVAKGAMQTGLNTTQNIATGT |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLIN4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Pig | 09/15/2020 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PLIN4 |
![Western Blot: Perilipin-4 Antibody [NBP2-13776] - Analysis in human adipose tissue .](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Western-Blot-NBP2-13776-img0024.jpg "Western Blot: Perilipin-4 Antibody [NBP2-13776] - Analysis in human adipose tissue .")
![Immunocytochemistry/Immunofluorescence: Perilipin-4 Antibody [NBP2-13776] - Staining of human cell line RT4 shows localization to plasma membrane, cytosol & lipid droplets. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13776-img0007.jpg "Immunocytochemistry/Immunofluorescence: Perilipin-4 Antibody [NBP2-13776] - Staining of human cell line RT4 shows localization to plasma membrane, cytosol & lipid droplets. Antibody staining is shown in green.")
![Immunohistochemistry-Frozen: Perilipin-4 Antibody [NBP2-13776] - Detection of PLIN4 in pig muscle tissue section. Antibody at 1:200 in PBS with 2% NGS. Secondary antibody at 1:1000 (Alexa Fluor 488 goat anti-rabbit IgG). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Frozen-NBP2-13776-img0025.jpg "Immunohistochemistry-Frozen: Perilipin-4 Antibody [NBP2-13776] - Detection of PLIN4 in pig muscle tissue section. Antibody at 1:200 in PBS with 2% NGS. Secondary antibody at 1:1000 (Alexa Fluor 488 goat anti-rabbit IgG). IHC-Fr image submitted by a verified customer review.")
![Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-13776-img0009.jpg "Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human breast shows moderate membranous positivity in adipocytes.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-13776-img0010.jpg "Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human breast shows moderate membranous positivity in adipocytes.")
![Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue shows moderate membranous positivity in adipocytes.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-13776-img0011.jpg "Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human adipose tissue shows moderate membranous positivity in adipocytes.")
![Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Perilipin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-13776-img0022.jpg "Immunohistochemistry-Paraffin: Perilipin-4 Antibody [NBP2-13776] - Staining of human colon shows no positivity in glandular cells as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
