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NRSF Antibody

Images

 
Biological Strategies: Western Blot: NRSF Antibody [NB100-757] - Detection of Human REST by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- REST used at a dilution of 1:1,000 (1ug/ml). Detection: DAB Counterstain: IHC ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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NRSF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
REST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:2500-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 29880481).
Reviewed Applications
Read 1 Review rated 3
using
NB100-757 in the following applications:

Publications
Read Publication using
NB100-757 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NRSF Antibody

  • Neural-restrictive silencer factor
  • NRSFneuron restrictive silencer factor
  • RE1-silencing transcription factor
  • X2 box repressor
  • XBRrepressor binding to the X2 box

Background

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-757
Species: Hu
Applications: WB, IHC, ChIP

Publications for NRSF Antibody (NB100-757)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Chemotaxis.


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Chemotaxis
(1)
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Human
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Review for NRSF Antibody (NB100-757) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-757:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot NRSF NB100-757
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3
reviewed by:
Verified Customer
WB Human 04/02/2015
View

Summary

ApplicationWestern Blot
Sample TestedLNCaP prostate cancer cells
SpeciesHuman
LotA2

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Secondary Antibodies

 

Isotype Controls

Additional NRSF Products

Array NB100-757

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Recent Reviews

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5
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1
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Verified Customer
04/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol REST
Entrez
Uniprot