![Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-Frozen-NBP1-30032-img0006.jpg "Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Bv, Ca, Fe, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clone | 3F4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Partially purified bovine 220-kDa disc rim protein. Accession # F1MWM0 |
| Epitope | PLHPRTAGASR (PMID: 9092582) |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ABCA4 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Although not confirmed, this product may be useful in Immunohistochemistry-Paraffin. Immunohistochemistry-Frozen was reported in scientific literature. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30889179). |
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| Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol |
| Preservative | No Preservative |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Feline | 09/25/2020 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for ABCA4 Antibody (NBP1-30032)Find related products by research area.
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![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0001.jpg "Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0003.jpg "Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in wild-type (ABCA4+/+) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.")
![Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0004.jpg "Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in heterozygous (ABCA4+/-) retina. PNA labels cone photoreceptors. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.")
![Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Western-Blot-NBP1-30032-img0002.jpg "Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.")
![Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ABCA4-Antibody-3F4-Immunohistochemistry-NBP1-30032-img0005.jpg "Knockout Validated: ABCA4 Antibody (3F4) [NBP1-30032] - Fluorescence histochemistry of ABCA4, cone photoreceptors, and autofluorescence in the canine retina. Fluorescence micrographs showing ABCA4 expression (red), FITC-conjugated peanut agglutinin (PNA, green), and DAPI nuclear staining (blue) in affected (ABCA4-/-) retina. PNA labels cone photoreceptors. Autofluorescence, indicative of lipofuscin accumulation, was seen in the ABCA4-/- RPE. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY license.")
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