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NRSF Antibody [Alexa Fluor® 750]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 750

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NRSF Antibody [Alexa Fluor® 750] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
REST
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NRSF Antibody [Alexa Fluor® 750]

  • Neural-restrictive silencer factor
  • NRSFneuron restrictive silencer factor
  • RE1-silencing transcription factor
  • X2 box repressor
  • XBRrepressor binding to the X2 box

Background

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional NRSF Products

Array NB100-757AF750

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Bioinformatics

Gene Symbol REST