What amino acid residues are recognized by the anti- Neurokinin 1 Receptor antibody NB300-119?

Name: NK1R Antibody - BSA Free
Brand: Novus Biologicals
SKU: NB300-119
Rating: 4.3 (3 reviews)

Question

Accepted Answer

The exact immunogen sequence is deemed proprietary by the lab, and we have not epitope mapped this particular antibody. Therefore we do not know exactly where NB300-119 binds to its target. However, the immunogen was derived from the N-terminus of the human Neurokinin 1 Receptor protein. If there is a specific region that you are interested in targeting, or excluding, please send your desired sequence information to our Technical Support team at technical@novusbio.com, for comparison to the immunogen sequence used to synthesize the antibody.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, GpSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Format	BSA Free
Concentration	1.0 mg/ml

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB300-119AF350

Alexa Fluor 405 NB300-119AF405

Alexa Fluor 488 NB300-119AF488

Alexa Fluor 532 NB300-119AF532

Alexa Fluor 594 NB300-119AF594

Alexa Fluor 647 NB300-119AF647

Alexa Fluor 700 NB300-119AF700

Alexa Fluor 750 NB300-119AF750

Allophycocyanin NB300-119APC

Allophycocyanin/Cy7 NB300-119APCCY7

Biotin NB300-119B

CoraFluor 1 NB300-119CL1

DyLight 350 NB300-119UV

DyLight 405 NB300-119V

DyLight 488 NB300-119G

DyLight 550 NB300-119R

DyLight 594 NB300-119DL594

DyLight 650 NB300-119C

DyLight 680 NB300-119FR

DyLight 755 NB300-119IR

FITC NB300-119F

HRP NB300-119H

Janelia Fluor 525 NB300-119JF525

Janelia Fluor 549 NB300-119JF549

Janelia Fluor 585 NB300-119JF585

Janelia Fluor 635 NB300-119JF635

Janelia Fluor 646 NB300-119JF646

Janelia Fluor 669 NB300-119JF669

PE NB300-119PE

PE/Atto594 NB300-119PEATT594

PE/Cy5.5 NB300-119PECY55

PE/Cy7 NB300-119PECY7

PerCP NB300-119PCP

mFluor Violet 450 SE NB300-119MFV450

mFluor Violet 500 SE NB300-119MFV500

mFluor Violet 610 SE NB300-119MFV610

Catalog# & Formulation	Size	Price
BSA Free NB300-119SS

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB300-119AF350
Alexa Fluor 405	NB300-119AF405
Alexa Fluor 488	NB300-119AF488
Alexa Fluor 532	NB300-119AF532
Alexa Fluor 594	NB300-119AF594
Alexa Fluor 647	NB300-119AF647
Alexa Fluor 700	NB300-119AF700
Alexa Fluor 750	NB300-119AF750
Allophycocyanin	NB300-119APC
Allophycocyanin/Cy7	NB300-119APCCY7
Biotin	NB300-119B
CoraFluor 1	NB300-119CL1
DyLight 350	NB300-119UV
DyLight 405	NB300-119V
DyLight 488	NB300-119G
DyLight 550	NB300-119R
DyLight 594	NB300-119DL594
DyLight 650	NB300-119C
DyLight 680	NB300-119FR
DyLight 755	NB300-119IR
FITC	NB300-119F
HRP	NB300-119H
Janelia Fluor 525	NB300-119JF525
Janelia Fluor 549	NB300-119JF549
Janelia Fluor 585	NB300-119JF585
Janelia Fluor 635	NB300-119JF635
Janelia Fluor 646	NB300-119JF646
Janelia Fluor 669	NB300-119JF669
PE	NB300-119PE
PE/Atto594	NB300-119PEATT594
PE/Cy5.5	NB300-119PECY55
PE/Cy7	NB300-119PECY7
PerCP	NB300-119PCP
mFluor Violet 450 SE	NB300-119MFV450
mFluor Violet 500 SE	NB300-119MFV500
mFluor Violet 610 SE	NB300-119MFV610

NK1R Antibody - BSA Free

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Publications for NK1R Antibody (NB300-119)(43)

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Immunogen	A peptide derived from the N-terminus region between amino acids 1-50 of human Neurokinin 1 Receptor. [UniProt# P25103]
Localization	Cell Membrane
Predicted Species	Guinea Pig (94%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	TACR1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.02% Sodium Azide
Concentration	1.0 mg/ml
Purity	Immunogen affinity purified

Publications using NB300-119	Applications	Species
Werner DF. Elucidating the Role of Alpha1-containing GABA(A) Receptors in Ethanol Action PhD Thesis University of Pittsburgh (ISBN 0549455426, 9780549455424). 2007-01-01 (WB, Mouse)	WB	Mouse
Zheng Y, Sang M, Liu F et al. Aprepitant inhibits the progression of esophageal squamous cancer by blocking the truncated neurokinin?1 receptor Oncology reports 2023-07-01 [PMID: 37203393] (WB, Human)	WB	Human
Restaino AC, Walz A, Vermeer SJ et al. Functional neuronal circuits promote disease progression in cancer Science advances 2023-05-10 [PMID: 37163587] (WB, IHC, Human) Details: IHC Dilution: 1:500; WB Dilution: 1:500	WB, IHC	Human
Ogawa, K;Khan, KN;Koshiba, A;Fujishita, A;Horiguchi, G;Teramukai, S;Itoh, K;Guo, SW;Mori, T; Association between tissue stress reaction and ACE2/TMPRSS2 expression in endometria of reproductive aged women before and during Covid-19 pandemic BMC women's health 2023-05-04 [PMID: 37142998]
Zhu E, Liu Y, Zhong M et al. Targeting NK-1R attenuates renal fibrosis via modulating inflammatory responses and cell fate in chronic kidney disease Frontiers in Immunology 2023-03-24 [PMID: 37033943] (IHC)	IHC
Brzozowska M, Romaniewicz M, Calka J, Jana B Effects of Substance P and Neurokinin A on the Contractile Activity of Inflamed Porcine Uterus International Journal of Molecular Sciences 2022-10-29 [PMID: 36361972] (WB, Porcine)	WB	Porcine
Guan M, Ying S, Wang Y. Increased expression of transient receptor potential channels and neurogenic factors associates with cough severity in a guinea pig model BMC Pulmonary Medicine 2021-12-01 [PMID: 34078339]
Xu X, Cai X, Liu X, Guo SW Possible involvement of neuropeptide and neurotransmitter receptors in Adenomyosis Reproductive biology and endocrinology : RB&E 2021-02-19 [PMID: 33602248] (IF/IHC, Human)	IF/IHC	Human
Morelli Ae, Sumpter Tl, Rojas-Canales Dm et Al. Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep 2020-03-10 [PMID: 32160549] (WB, Mouse)	WB	Mouse
Talbot S, Doyle B, et al. Vagal sensory neurons drive mucous cell metaplasia. J Allergy Clin Immunol 2020-06-01 [PMID: 31954778] (FLOW, Mouse)	FLOW	Mouse
Zhou Y, Wang M, Tong Y et al. miR-206 Promotes Cancer Progression by Targeting Full-Length Neurokinin-1 Receptor in Breast Cancer Technol. Cancer Res. Treat. 2019-01-01 [PMID: 31506061] (WB, IF/IHC, Human, Mouse)	WB, IF/IHC	Human, Mouse
Li Q, Wu X, Yang Y et al. Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice Int. J. Biochem. Cell Biol. 2018-12-26 [PMID: 30593954] (WB, Mouse)	WB	Mouse
Bubak AN, Como CN, Blackmon AM et al. Varicella Zoster Virus Induces Nuclear Translocation of the Neurokinin-1 Receptor, Promoting Lamellipodia Formation and Viral Spread in Spinal Astrocytes J. Infect. Dis. 2018-05-19 [PMID: 29788447] (WB)	WB
Wang Dong, Tawfik Vivianne L, Corder Gregory et al. Functional Divergence of Delta and Mu Opioid Receptor Organization in CNS Pain Circuits. Genome Medicine 2018-04-04 [PMID: 29576387] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang Z, Zheng W, Xie H et al. Up-regulated expression of substance P in CD8(+) T cells and NK1R on monocytes of atopic dermatitis. J Transl Med 2017-05-01 [PMID: 28460633] (Mouse)		Mouse
Henssen AG, Odersky A, Szymansky A et al. Targeting tachykinin receptors in neuroblastoma. Oncotarget. 2016-11-18 [PMID: 27888795] (WB, Human)	WB	Human
Zhou Y, Zuo D, Wang M et al. Effect of truncated neurokinin-1 receptor expression changes on the interaction between human breast cancer and bone marrow-derived mesenchymal stem cells. Genes Cells. 2014-09-01 [PMID: 25130457] (WB, Human)	WB	Human
Fujisawa D, Kashiwakura JI, Kita H et al. Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria. J. Allergy Clin. Immunol. 2014-06-19 [PMID: 24954276] (FLOW, Human) Details: NK-1R on the cell surface of Human skin/lung-derived cultured Mast cells (MCs) was analyzed by using flow cytometric analysis. MCs fixed in 4% paraformaldehyde ( 20 minutes at 4°C) were incubated for 30 minutes at 4°C with anti–NK-1R polyclonal antibody and detected via biotinylated goat anti-rabbit IgG - phycoerythrin-conjugated streptavidin using the FACSCalibur /analyzed with FlowJo software. The MCs were found to be negative for the signal whereas the positive control Jurkat cells expressed NK-1R ( Figure 1D)	FLOW	Human
Zhou Y, Zhao L, Xiong T et al. Roles of full-length and truncated neurokinin-1 receptors on tumor progression and distant metastasis in human breast cancer. Breast Cancer Res Treat 2013-07-01 [PMID: 23807418] (IHC-P, Human)	IHC-P	Human
Schwartz L, Spitsin SV, Meshki J et al. Substance P enhances HIV-1 infection in human fetal brain cell cultures expressing full-length neurokinin-1 receptor. J Neurovirol 2013-06-14 [PMID: 23765222] (ICC/IF, Mouse)	ICC/IF	Mouse
Humes, D J, Simpson, J, Smith, J et al. Visceral hypersensitivity in symptomatic diverticular disease and the role of neuropeptides and low grade inflammation Neurogastroenterology & Motility 2012-01-01 [PMID: 22276853] (IF/IHC, Human)	IF/IHC	Human
Altawil R, Lystrom J, El-Nour H. Kinetics of neuronal contribution during the development of a contact allergic reaction. Arch Dermatol Res 2012-05-01 [PMID: 22198823] (IF/IHC, Human) Details: Using the DyLight 650 conjugated version of NB300-119, catalog number NB300-119C.	IF/IHC	Human
Janssen MJ, Yasuda RP, Vicini S. GABA(A) Receptor Beta3 Subunit Expression Regulates Tonic Current in Developing Striatopallidal Medium Spiny Neurons. Front Cell Neurosci 2011-07-28 [PMID: 21847370] (WB, Mouse)	WB	Mouse
Nayate A et al. Cardiac damage after lesions of the nucleus tractus solitarii. Am J Physiol Regul Integr Comp Physiol;296(2):R272-9. 2009-02-01 [PMID: 19020288] (IF/IHC, Rat)	IF/IHC	Rat
Massouh M, Wallman MJ, Pourcher E, Parent A. The fate of the large striatal interneurons expressing calretinin in Huntington's disease. Neurosci Res 2008-12-01 [PMID: 18801393] (Human)		Human
Schmidtko A et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci;28(34):8568-76. 2008-08-20 [PMID: 18716216] (IF/IHC, Mouse)	IF/IHC	Mouse
Andersson G, Danielson P, Alfredson H, Forsgren S. Presence of substance P and the neurokinin-1 receptor in tenocytes of the human Achilles tendon. Regul Pept 2008-10-09 [PMID: 18394729] (Human)		Human
Grimsholm O, Rantapaa-Dahlqvist S, Dalen T, Forsgren S. Observations favouring the occurrence of local production and marked effects of bombesin/gastrin-releasing peptide in the synovial tissue of the human knee joint--comparisons with substance P and the NK-1 receptor. Neuropeptides 2008-04-01 [PMID: 18289674] (Human)		Human
Ferguson C, Hardy SL, Werner DF et al. New insight into the role of the beta3 subunit of the GABAA-R in development, behavior, body weight regulation, and anesthesia revealed by conditional gene knockout. BMC Neurosci. 2007-10-10 [PMID: 17927825] (WB, Mouse)	WB	Mouse
Andersson G, Danielson P, Alfredson H et al. Nerve-related characteristics of ventral paratendinous tissue in chronic Achilles tendinosis. Knee Surg Sports Traumatol Arthrosc. 2007-10-01 [PMID: 17604979] (IHC-Fr, Human)	IHC-Fr	Human
Hazlett, LD et al. Spantide I decreases type I cytokines, enhances IL-10, and reduces corneal perforation in susceptible mice after Pseudomonas aeruginosa infection. Invest Ophthalmol Vis Sci;48(2):797-807. 2007-02-01 [PMID: 17251480] (IF/IHC, Mouse)	IF/IHC	Mouse
Levesque M, Wallman MJ, Parent R et al. Neurokinin-1 and neurokinin-3 receptors in primate substantia nigra. Neurosci Res 2007-03-01 [PMID: 17134780]
Fong, AY, Potts, JT. Neurokinin-1 receptor activation in Botzinger complex evokes bradypnoea. J Physiol;575(Pt 3):869-85. 2006-09-15 [PMID: 16825299] (IF/IHC, Rat)	IF/IHC	Rat
Levesque M, Parent R, Parent A. Cellular and subcellular localization of neurokinin-1 and neurokinin-3 receptors in primate globus pallidus. Eur J Neurosci 2006-05-01 [PMID: 16817879]
Borghese CM, Werner DF, Topf N et al. An isoflurane- and alcohol-insensitive mutant GABA(A) receptor alpha(1) subunit with near-normal apparent affinity for GABA: characterization in heterologous systems and production of knockin mice. J Pharmacol Exp Ther. 2006-10-01 [PMID: 16807363] (WB, Mouse)	WB	Mouse
Lai JP, Ho WZ, Kilpatrick LE et al. Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation. Proc Natl Acad Sci U S A 2006-05-16 [PMID: 16675550] (ICC/IF, Human)	ICC/IF	Human
Persson-Sjogren S, Lejon K, Holmberg D, Forsgren S. Expression of the NK-1 receptor on islet cells and invading immune cells in the non-obese diabetic mouse. J Autoimmun 2005-06-01 [PMID: 15869864] (Mouse)		Mouse
Jonsson M, Norrgard O, Forsgren S. Substance P and the neurokinin-1 receptor in relation to eosinophilia in ulcerative colitis. Peptides 2005-05-01 [PMID: 15808910] (Human)		Human
Forsgren S, Danielson P, Alfredson H. Vascular NK-1 receptor occurrence in normal and chronic painful Achilles and patellar tendons: studies on chemically unfixed as well as fixed specimens. Regul Pept 2005-03-30 [PMID: 15664664] (Human)		Human
Benarroch, E. Depletion of ventromedullary NK-1 receptor-immunoreactive neurons in multiple system atrophy. Brain;126(Pt 10):2183-90. 2003-10-01 [PMID: 12902309] (IF/IHC, Human)	IF/IHC	Human
Shrikhe, S et al. NK-1 receptor gene expression is related to pain in chronic pancreatitis. Pain;91(3):209-17. 2001-04-01 [PMID: 11275376] (IF/IHC, Human)	IF/IHC	Human
Tooney, PA et al. Increased tachykinin NK(1) receptor immunoreactivity in the prefrontal cortex in schizophrenia. Biol Psychiatry;49(6):523-7. 2001-03-15 [PMID: 11257237] (IF/IHC, Human)	IF/IHC	Human
Tooney, Paul A et al. Localisation of tachykinin NK1 and NK3 receptors in the human prefrontal and visual cortex. Neurosci Lett;283(3):185-8. 2000-04-14 [PMID: 10754218] (IF/IHC, Human)	IF/IHC	Human
Show All 43 Publications. Collapse Publications.

	Patricia Carulla Martí 06/28/2023
Application:	ICC
Species:	Human

	Megan Janssen-Schroeder 12/27/2011
Application:	WB
Species:	Mouse