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NK3R/TACR3/Neurokinin B Receptor Antibody

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Product Details

Summary
Reactivity Mu, Rt, Gp, Sh, Hu(-)Species Glossary
Applications ICC/IF, IHC, IHC, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NK3R/TACR3/Neurokinin B Receptor Antibody Summary

Immunogen
A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin. [UniProt# P16177]
Localization
In immunohistochemical experiments staining of the neuronal cell bodies and fibers is seen and staining is believed to be localized to the plasma membrane.
Specificity
NB300-102 is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACR3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 25496429)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500. Use reported in scientific literature (PMID 30565563)
Application Notes
It is not useful for Western blot.
Control Peptide
NK3R/TACR3/Neurokinin B Receptor Protein (NB300-102PEP)
Publications
Read Publications using
NB300-102 in the following applications:

Reactivity Notes

Rat, mouse and guinea pig in brain, spinal cord and ileum. Customer feedback suggests that human reactivity is negative. Sheep reactivity reported in scientific literature (PMID: 19912479).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for NK3R/TACR3/Neurokinin B Receptor Antibody

  • Neurokinin B Receptor
  • neurokinin beta receptor
  • Neuromedin-K Receptor
  • NK-3 receptor
  • NK3R
  • NK-3R
  • NK3RMGC148061
  • NKR
  • TAC3R
  • TAC3RL
  • tachykinin receptor 3MGC148060
  • TACR3

Background

Neurokinins belong to tachykinin family of signalling peptides and the best known human tachykinins are neurokinin B, (encoded by TAC3) and substance P (SP) as well as neurokinin A (NKA), (both encoded by the same TAC1 gene). Neurokinins are widely expressed in CNS, and exert effects on target cells through action at three closely related Gq-coupled neurokinin receptors: NK1R, NK2R, NK3R: encoded in humans by TACR1, TACR2 and TACR3 respectively. None of these neurokinin receptors are entirely specific for SP, NKA or NKB, however, they do show some degree of selectivity, and TACR3/NK3R (neurokinin B receptor or neuromedin-K receptor) behaves as cognate receptor for NKB in vivo. NK3R are predominantly expressed in neurons of PNS/CNS and in particular, in many of forebrain areas, such as frontal, parietal and cingulate cortices, and basal ganglia structures implicated in psychiatric disease states, and can modulate monoaminergic and amino acid neurotransmission within these structures. Neurokinin B and NK3R are also expressed in placenta, kidney, skeletal muscle and lung tissues, and had been implicated in diverse processes, including water homeostasis, pulmonary inflammation, cognition, and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102)(37)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Sheep.

We have publications tested in 5 applications: ICC, ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl.


Filter By Application
ICC
(1)
ICC/IF
(4)
IF/IHC
(9)
IHC-Fr
(5)
IHC-FrFl
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
Rat
(10)
Guinea Pig
(1)
Sheep
(4)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB300-102 Applications Species
Kodani Y, Kawata M, Suga H et al. Characterization of Hypothalamic MCH Neuron Development in a 3D Differentiation System of Mouse Embryonic Stem Cells eNeuro [PMID: 35437265]
Chen SL, Liu YG, Zhou YT et al. Morphological and Functional Evidence of Sexual Dimorphism in the Retrochiasmatic Area Population of NK3R-containing Neurons in Sheep Thesis (IHC-FrFl, Sheep) IHC-FrFl Sheep
Persephone A Miller, Jesukhogie G Williams-Ikhenoba, Aditi S Sankhe, Brendan H Hoffe, Melissa J Chee Neuroanatomical, electrophysiological, and morphological characterization of melanin-concentrating hormone cells coexpressing cocaine- and amphetamine-regulated transcript. The Journal of comparative neurology 2024-02-14 [PMID: 38335050]
Edvinsson JC, Reducha PV, Sheykhzade M et al. Neurokinins and their receptors in the rat trigeminal system: Differential localization and release with implications for migraine pain Molecular pain 2021-12-13 [PMID: 34898306] (IHC-Fr, Rat) IHC-Fr Rat
Ashley N Lindo, Jennifer F Thorson, Michelle N Bedenbaugh, Richard B McCosh, Justin A Lopez, Samantha A Young, Lanny J Meadows, Elizabeth C Bowdridge, Chrysanthi Fergani, Bradley A Freking, Michael N Lehman, Stanley M Hileman, Clay A Lents Localization of kisspeptin, NKB, and NK3R in the hypothalamus of gilts treated with the progestin altrenogest. Biology of reproduction 2022-01-03 [PMID: 34037695]
Lopez, J A, Bowdridge, E C Et al. Morphological and functional evidence for sexual dimorphism in neurokinin B signalling in the retrochiasmatic area of sheep. J Neuroendocrinol 2020-07-01 [PMID: 32572994] (IF/IHC, Mouse) IF/IHC Mouse
Yin C, Ishii T, Kaneda M Two Types of Cl Transporters Contribute to the Regulation of Intracellular Cl Concentrations in ON- and OFF-type Bipolar Cells in the Mouse Retina Neuroscience 2020-06-10 [PMID: 32531472] (IF/IHC, Mouse) IF/IHC Mouse
Goodman R L, He W et al. Evidence That the LH Surge in Ewes Involves Both Neurokinin B-Dependent and -Independent Actions of Kisspeptin. Endocrinology 2019-01-12 [PMID: 31599937] (IF/IHC, Sheep) IF/IHC Sheep
Sakata D, Uruno T et al. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol 2019-01-10 [PMID: 31405606] (IF/IHC, Mouse) IF/IHC Mouse
Fergani C, Leon S, Padilla SL et al. NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice Elife 2018-12-19 [PMID: 30565563] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 37 Publications.

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Product General Protocols

View specific protocols for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering about the Rabbit polyclonal antibody to Neurokinin-3-receptor, catalog #NB300-102. I am wondering about its information on the immunogen. You state that, NB300-102 was raised against a synthetic peptide at the c-terminus of rat Nk-3 conjugated to bovine thyroglobulin. I was wondering if you could tell me exactly which amino acids that this does correspond to? I am interested in comparing it to the Rabbit anti-Nk3R used for western blot, catalog#R-103-100, which states that it corresponds to the aa:434-452 of the absolute c-terminal of rat Nk3R protein.
    • The immunogen for this antibody is between amino acids 375 and 452 of rat NK3R. Any further information regarding the immunogen is considered proprietary.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TACR3
Entrez
Uniprot