![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0003.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody")
| Reactivity | Hu, Mu, Rt, MarSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human NDC80. The exact sequence is proprietary. |
| Modification | p Ser55 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NDC80 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![NDC80 [p Ser55] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "NDC80 [p Ser55] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for NDC80 Antibody (NBP3-13726)Find related products by research area.
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| Gene Symbol | NDC80 |
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 (phospho Ser 55) antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0001.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 (phospho Ser 55) antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0002.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)")
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13726_rabbit-ndc80-p-ser-55-pab-9102024205664.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
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![Flow Cytometry Integrin beta 2/CD18 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1730_human-integrin-beta-2-cd18-affinity-purified-polyclonal-ab-flow-cytometry-7220258626..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")