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![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0016.jpg "Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Western-Blot-NB100-338-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - Mouse anti-Hec1 and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in fixed human cell lines of RPE1, H4, and 42MGBA. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - IF analysis of NDC80 in PTK1 cells.Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize/NDC80-Antibody-9G3-23-Immunocytochemistry-Immunofluorescence-NB100-338-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024205614.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-91020242024619.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-9102024200109.jpg)
![Immunocytochemistry/ Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - NDC80 antibody [9G3.23] detects NDC80 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDC80 stained by NDC80 antibody [9G3.23] (NB100-338) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-910202420134060.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hec1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nb100-338_mouse-monoclonal-ndc80-antibody-9g3-23-1610202419405719.jpg)
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0003.jpg "Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Purified 6his-NDC80 was subject to a kinase assay with recombinant Aurora B and gamma-p32-labelled ATP. The resultant phospho-NDC80 were detected by measuring radioactive signal and by WB analysis with pSer55 antibody")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NDC80 [p Ser55] Antibody [NBP3-13726] - Immunofluorescent staining of NDC80 phospho Ser55 in cells treated with NDC80 siRNA (bottom) or control siRNA (top)](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13726-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 (phospho Ser 55) antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0001.jpg)
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - GFP-tagged NDC80 wild type, S55A or 6A (Ser55 and five other Ser/Thr residues changed to Ala) mutant was ectopically expressed in HeLa cells and the cell lysate was used for WB analysis with the NDC80 pSer55 antibody (diluted at 1:500)](http://images.novusbio.com/fullsize/NDC80-[p-Ser55]-Antibody-Western-Blot-NBP3-13726-img0002.jpg)
![Western Blot: NDC80 [p Ser55] Antibody [NBP3-13726] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NDC80 antibody (NBP3-13726) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13726_rabbit-ndc80-p-ser-55-pab-9102024205664.jpg)
![Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NB100-1100-img0003.jpg "Western Blot: NDC80 Antibody [NB100-1100] - Staining (0.5 ug/ml) of Hela lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0002.jpg)
![Immunohistochemistry-Paraffin: NDC80 Antibody [NB100-1100] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunohistochemistry-Paraffin-NB100-1100-img0001.jpg)
![Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Western-Blot-H00010403-M01-img0004.jpg "Western Blot: NDC80 Antibody (1A10) [H00010403-M01] - KNTC2 monoclonal antibody (M01), clone 1A10 Analysis of KNTC2 expression in Hela S3 NE.")
![Sandwich ELISA: NDC80 Antibody (1A10) [H00010403-M01] - Detection limit for recombinant GST tagged KNTC2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NDC80-Antibody-1A10-Sandwich-ELISA-H00010403-M01-img0003.jpg)
![Western Blot: NDC80 Antibody [NBP2-56920] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/NDC80-Antibody-Western-Blot-NBP2-56920-img0002.jpg "Western Blot: NDC80 Antibody [NBP2-56920] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody [NBP2-56920] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDC80-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56920-img0001.jpg)
![Western Blot: NDC80 Antibody (SR2187) [NBP3-21773] - Western blot analysis using NBP3-21773 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21773_rabbit-ndc80-mab-sr2187-57202315521811.jpg "Western Blot: NDC80 Antibody (SR2187) [NBP3-21773] - Western blot analysis using NBP3-21773 in Jurkat cell lysate.")
![SDS-Page: Recombinant Human NDC80 Protein [H00010403-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010403-P01-1.jpg "SDS-Page: Recombinant Human NDC80 Protein [H00010403-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121] - Western blot analysis of extracts of various cell lines, using NDC80 Rabbit mAb (NBP3-16121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/NDC80-Antibody-4W2Y2-Western-Blot-NBP3-16121-img0001.jpg "Western Blot: NDC80 Antibody (4W2Y2) [NBP3-16121] - Western blot analysis of extracts of various cell lines, using NDC80 Rabbit mAb (NBP3-16121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32419_rabbit-ndc80-mab-je31-10-278202415295591.jpg "Western Blot: NDC80 Antibody (JE31-10) [NBP3-32419] - Western blot analysis of NDC80 on different lysates with Rabbit anti-NDC80 antibody (NBP3-32419) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32419) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")