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Myosin Heavy Chain 1 Antibody - Azide and BSA Free

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Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Analysis of extracts of various cell lines, using MYH1 antibody at 1:410 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10038 ...read more
Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] -analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] -Immunofluorescence analysis of paraffin-embedded mouse heart using MYH1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat ...read more
Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] -Immunofluorescence analysis of paraffin-embedded Human heart using MYH1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat ...read more
Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Human skeletal muscle using MYH1 Rabbit pAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

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Myosin Heavy Chain 1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3). MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYH1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:100 - 1:500
Theoretical MW
223 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for Myosin Heavy Chain 1 Antibody - Azide and BSA Free

  • DFNA4
  • FLJ13881
  • FLJ43092
  • KIAA2034DKFZp667A1311
  • MHC16
  • MYH1
  • MYH14 variant protein
  • MYH17
  • MyHC-2x
  • MyHC-IIx/d
  • Myosin Heavy Chain 1
  • Myosin heavy chain 14
  • Myosin heavy chain, non-muscle IIc
  • myosin
  • myosin, heavy chain 14
  • myosin, heavy chain 14, non-muscle
  • myosin, heavy polypeptide 14
  • myosin-14
  • NMHC II-C
  • NMHC-II-C
  • Non-muscle myosin heavy chain IIc
  • nonmuscle myosin heavy chain II-C

Background

The Myosin family includes a number of ATP-driven motor proteins involved in muscle contration and actin-dependent cellular motility. Although they were originally thought to be limited to muscle tissues, new genes sharing basic myosin superfamily properties have since been identifed nearly all eukaryotic cell types. The myosin proteins are spearated into 18 different classes, which have been found to be highly conserved across species. Myosin antibodies are commonly used for cell motility, ATP hydrolysis and muscle studies, as well as loading controls.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MYH1