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MYH4 Antibody - BSA Free

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Western Blot: MYH4 Antibody [NBP3-05634] - Western blot analysis of extracts of C2C12 cells, using MYH4 antibody (NBP3-05634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. ...read more
Immunohistochemistry: MYH4 Antibody [NBP3-05634] - Immunofluorescence analysis of mouse skeletal muscle using MYH4 antibody (NBP3-05634) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blot: MYH4 Antibody [NBP3-05634] - Western blot analysis of extracts of Rat skeletal muscle, using MYH4 antibody (NBP3-05634) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry-Paraffin: MYH4 Antibody [NBP3-05634] - Mouse heart using MYH4 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry-Paraffin: MYH4 Antibody [NBP3-05634] - Mouse skeletal muscle using MYH4 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry-Paraffin: MYH4 Antibody [NBP3-05634] - Rat heart using MYH4 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry-Paraffin: MYH4 Antibody [NBP3-05634] - Rat skeletal muscle using MYH4 antibody at dilution of 1:150 (40x lens).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

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Catalog# & Formulation Size Price
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MYH4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1500-1600 of human MYH4 (NP_060003.2). ETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVES
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYH4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:100 - 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Alternate Names for MYH4 Antibody - BSA Free

  • MYH4
  • MyHC-2b
  • MyHC-IIb
  • myosin heavy chain 4
  • myosin heavy chain IIb
  • myosin heavy chain, skeletal muscle, fetal
  • myosin, heavy chain 4, skeletal muscle
  • myosin, heavy polypeptide 4, skeletal muscle
  • myosin-4

Background

MYH4, also known as Myosin-4, is a 1939 amino acid protein that is 223 kDa, predominantly found in myocytes mediates plus-ended movement along microfilaments and involved in muscle contraction through cyclic interactions with actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations. Current research is being performed on several diseases and disorders including congestive heart failure, yaws, myocarditis, hermaphroditism, liver disease, pharyngitis, and alcoholism. This protein is involved in cell adhesion integrin-mediated cell adhesion and migration, cell adhesion tight junctions, cytoskeleton remodeling regulation of actin cytoskeleton by Rho GTPases, immune response CCR3 signaling in eosinophils, development MAG-dependent inhibition of neurite outgrowth, RhoA, antioxidant action of vitamin-C, transendothelial migration of leukocytes, actin nucleation by ARP-WASP complex, epithelial adherens junctions, inhibitory action of lipoxins on neutrophil migration, membrane trafficking, translocation of GLUT4 to the plasma membrane, translocation of GLUT4 vesicle and docking at the plasma membrane, and Viral myocarditis pathways forming interactions with ESR2, NTHL1, S100A4, USP15, C12orf44, CNBP, ACTB, and over 30 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MYH4 Products

Array NBP3-05634

Research Areas for MYH4 Antibody (NBP3-05634)

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Bioinformatics

Gene Symbol MYH4