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Myogenin Antibody (671037)

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 (1 Review)
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Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myogenin at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG ...read more
Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
671037
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB66861-SP
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Myogenin Antibody (671037) Summary

Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Specificity
Detects human Myogenin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB66861 in the following applications:
Publications
Read Publications using
MAB66861 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myogenin Antibody (671037)

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Background

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Myogenin Antibody
(671037)
MAB66861
Myogenin Antibody (671037)
Species: Hu
Applications: WB, ICC/IF

Publications for Myogenin Antibody (MAB66861)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Simple Western.


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Simple Western
(1)
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(1)
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Review for Myogenin Antibody (MAB66861) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB66861:
Filter by Applications
Simple Western
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Simple Western Myogenin MAB66861
Enlarge 		4
reviewed by:
Zachary Patinkin
 Simple Western Human 02/25/2015
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Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate; 3T3-L1 cell lysate
SpeciesHuman
LotCFUU0114021

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Recent Reviews

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1
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Simple Western Myogenin MAB66861
Enlarge
 Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human
read this review

Bioinformatics

Gene Symbol MYOG
Uniprot