![Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)](http://images.novusbio.com/fullsize/MYH6-Antibody-3-48-Western-Blot-NB300-284-img0004.jpg "Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)")
| Reactivity | Hu, Mu, Rt, Ca, Bv, RbSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | 3-48 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Human ventricular myosin |
| Specificity | This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains. |
| Predicted Species | Bovine (97%), Rabbit (91%). Backed by our 100% Guarantee. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MYH6 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Please only use IgG speciific secondaries with this product. Fab region specific secondaries may not detect this antibody. ICC/IF reactivity reported in (PMID: 23577316). |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized protein G purified in PBS pH7.4 (Full IgG) |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 11/25/2014 |
Summary
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reviewed by:
Verified Customer |
WB | Rat | 12/30/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for MYH6 Antibody (NB300-284)Find related products by research area.
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| Verified Customer 11/25/2014 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 12/30/2011 |
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| Application: | WB | |
| Species: | Rat |
![Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.](http://images.novusbio.com/fullsize/MYH6-Antibody-3-48-Immunohistochemistry-Paraffin-NB300-284-img0005.jpg "Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.")
![Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHCalpha and MyHCbeta isoforms. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2011/634253/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MYH6-Antibody-3-48-Western-Blot-NB300-284-img0006.jpg "Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHCalpha and MyHCbeta isoforms. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/bmri/2011/634253/), licensed under a CC-BY license.")
![Western Blot: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates & extracts of adult euthyroid (EU), hypothyroid (HY), & hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHC alpha & MyHC beta isoforms (a) or by BA.G5 antibody, which was solely specific for cardiac MyHC alpha isoform (b). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22187528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-284_mouse-monoclonal-myh6-antibody-3-48-31020241535690.jpg "Western Blot: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates & extracts of adult euthyroid (EU), hypothyroid (HY), & hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHC alpha & MyHC beta isoforms (a) or by BA.G5 antibody, which was solely specific for cardiac MyHC alpha isoform (b). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22187528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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