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Myosin Heavy Chain Antibody (MF20) [Unconjugated]

Images

 
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells ...read more
Myosin Heavy Chain was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room ...read more
Myosin Heavy Chain was detected in perfusion fixed frozen sections of mouse skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 5 µg/mL for 1 hour at room temperature ...read more
Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of ...read more
Generation of DM1-iPSCs and their differentiation.(A) The strategy of our study: patient iPSCs were passed and differentiated at three different passage numbers into CMs or neurons giving 9 samples (left), or had a ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
MF20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price
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Myosin Heavy Chain Antibody (MF20) [Unconjugated] Summary

Immunogen
Chicken pectoralis-derived Myosin
Specificity
Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MYH14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot
Reviewed Applications
Read 2 Reviews rated 4
using
MAB4470 in the following applications:

Publications
Read Publications using
MAB4470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myosin Heavy Chain Antibody (MF20) [Unconjugated]

  • DFNA4
  • FLJ13881
  • FLJ43092
  • KIAA2034DKFZp667A1311
  • MHC16
  • MYH14 variant protein
  • MYH17
  • MYHC
  • Myosin heavy chain 14
  • Myosin Heavy Chain
  • Myosin heavy chain, non-muscle IIc
  • myosin
  • myosin, heavy chain 14
  • myosin, heavy chain 14, non-muscle
  • myosin, heavy polypeptide 14
  • myosin-14
  • NMHC II-C
  • NMHC-II-C
  • Non-muscle myosin heavy chain IIc
  • nonmuscle myosin heavy chain II-C

Background

Skeletal muscle Myosin or myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin Heavy Chain Antibody (MAB4470)(165)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chicken, Fish - Danio rerio (Zebrafish), Goat, Transgenic Mouse, Xenograft.

We have publications tested in 12 applications: Bioassay, Flow Cytometry, ICC, ICC/IF, IF, IHC, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(2)
ICC
(52)
ICC/IF
(2)
IF
(1)
IHC
(14)
IHC-P
(2)
Immunocytochemistry
(7)
Immunohistochemistry
(2)
Immunoprecipitation
(1)
Simple Western
(1)
Western Blot
(46)
All Applications
Filter By Species
Human
(27)
Mouse
(69)
Rat
(2)
Chicken
(2)
Fish - Danio rerio (Zebrafish)
(1)
Goat
(3)
Transgenic Mouse
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 165. Show All 165 Publications.
Publications using MAB4470 Applications Species
Yu Liu, S;Chen, LK;Chung, YT;Chen, CW;Wu, GL;Chang, YC;Chen, PR;Chang, YI;Lin, HF;Wu, LY;Juan, CC; Glucosamine inhibits myoblast proliferation and differentiation, and stimulates myotube atrophy through distinct signal pathways The Journal of nutritional biochemistry 2024-09-07 [PMID: 39251145] (Immunocytochemistry, Mouse) Immunocytochemistry Mouse
Maejima, Y;Nah, J;Aryan, Z;Zhai, P;Sung, EA;Liu, T;Takayama, K;Moghadami, S;Sasano, T;Li, H;Sadoshima, J; Mst1-mediated phosphorylation of FoxO1 and C/EBP-? stimulates cell-protective mechanisms in cardiomyocytes Nature communications 2024-07-25 [PMID: 39060225] (Immunocytochemistry, Rat) Immunocytochemistry Rat
Gao, H;Huang, X;Cai, Z;Cai, B;Wang, K;Li, J;Kuang, J;Wang, B;Zhai, Z;Ming, J;Cao, S;Qin, Y;Pei, D; Generation of musculoskeletal cells from human urine epithelium-derived presomitic mesoderm cells Cell & bioscience 2024-07-15 [PMID: 39010176] (Flow Cytometry, Immunohistochemistry, Human, Mouse) Flow Cytometry, Immunohistochemistry Human, Mouse
Lee, B;Park, Y;Lee, Y;Kwon, S;Shim, J; Triptolide, a Cancer Cell Proliferation Inhibitor, Causes Zebrafish Muscle Defects by Regulating Notch and STAT3 Signaling Pathways International journal of molecular sciences 2024-04-25 [PMID: 38731894] (Immunohistochemistry, Immunocytochemistry, Mouse, Fish - Danio rerio (Zebrafish)) Immunohistochemistry, Immunocytochemistry Mouse, Fish - Danio rerio (Zebrafish)
Fukushima, T;Hasegawa, Y;Kuse, S;Fujioka, T;Nikawa, T;Masubuchi, S;Sakakibara, I; PHF2 regulates sarcomeric gene transcription in myogenesis PloS one 2024-05-03 [PMID: 38701072] (Immunocytochemistry, Mouse) Immunocytochemistry Mouse
Sun, Y;Ramesh, V;Wei, F;Locasale, JW; Methionine availability influences essential H3K36me3 dynamics during cell differentiation bioRxiv : the preprint server for biology 2023-11-22 [PMID: 38045360] (Western Blot, Mouse) Western Blot Mouse
Sarsons, CD;Gilham, D;Tsujikawa, LM;Wasiak, S;Fu, L;Rakai, BD;Stotz, SC;Carestia, A;Sweeney, M;Kulikowski, E; Apabetalone, a Clinical-Stage, Selective BET Inhibitor, Opposes DUX4 Target Gene Expression in Primary Human FSHD Muscle Cells Biomedicines 2023-09-30 [PMID: 37893058] (ICC, Human) ICC Human
Tetsuhiro Kakimoto, Akira Ogasawara, Kiyoshi Ishikawa, Takashi Kurita, Kumiko Yoshida, Shuichi Harada et al. A Systemically Administered Unconjugated Antisense Oligonucleotide Targeting DUX4 Improves Muscular Injury and Motor Function in FSHD Model Mice Biomedicines 8/22/2023 [PMID: 37760780]
Yoshioka, Y;Oishi, S;Onoda, K;Shibata, K;Miyoshi, N; Diosgenin prevents dexamethasone-induced myotube atrophy in C2C12?cells Archives of biochemistry and biophysics 2023-09-16 [PMID: 37722527] (Western Blot, ICC, Mouse) Western Blot, ICC Mouse
Aeyung Kim, Sang-Min Park, No Soo Kim, Haeseung Lee Ginsenoside Rc, an Active Component of Panax ginseng, Alleviates Oxidative Stress-Induced Muscle Atrophy via Improvement of Mitochondrial Biogenesis Antioxidants (Basel) 8/7/2023 [PMID: 37627571]
Show All 165 Publications.

Reviews for Myosin Heavy Chain Antibody (MAB4470) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB4470:
Filter by Applications
ICC
(1)
Simple Western
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Patricia Carulla Martí
ICC Human 06/19/2023
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary human myocytes
SpeciesHuman
LotCAEI0817071
Simple Western Myosin Heavy Chain MAB4470
Enlarge
4
reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
View

Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate; 3T3-L1 cell lysate
SpeciesHuman
LotCAEI0314061

Product General Protocols

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FAQs for Myosin Heavy Chain Antibody (MAB4470) (0)

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Patricia Carulla Martí
06/19/2023
Application: ICC
Species: Human

Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol MYH14