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Muscleblind-like 1 Antibody

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Western Blot: Muscleblind-like 1 Antibody [NBP2-19445] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: Muscleblind-like 1 Antibody [NBP2-19445] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Muscleblind-like 1 Antibody [NBP2-19445] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Muscleblind-like 1 stained by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Muscleblind-like 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Muscleblind-like 1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (100%), Rat (92%), Chicken (98%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MBNL1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 1:500-1:3000
Application Notes
IP Assay dependent
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Muscleblind-like 1 Antibody

  • DKFZp686P06174
  • EXP40
  • EXP42
  • EXPKIAA0428EXP35
  • MBNL
  • muscleblind (Drosophila)-like
  • muscleblind-like (Drosophila)
  • muscleblind-like protein 1
  • Triplet-expansion RNA-binding protein

Background

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-19445
Muscleblind-like 1 Antibody
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Applications: WB, ICC/IF, IP
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol MBNL1
Uniprot