![Western Blot: MEF2D Antibody [NBP1-80567] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80566, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/MEF2D-Antibody-Western-Blot-NBP1-80567-img0001.jpg "Western Blot: MEF2D Antibody [NBP1-80567] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody NBP1-80566, which recognizes an upstream epitope.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen this antibody was made to, maps to a region between residue 475 and 521 of human Myocyte Enhancer Factor 2D using the numbering given in entry NP_005911.1 (GeneID 4209). |
| Predicted Species | Rat (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MEF2D |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-80567 | Applications | Species |
|---|---|---|
| G�nczi M, Teixeira JMC, Barrera-Vilarmau S et al. Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis Nature communications 2023-03-10 [PMID: 36898987] (Chromatin Immunoprecipitation (ChIP), Mouse) | Chromatin Immunoprecipitation (ChIP) | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for MEF2D Antibody (NBP1-80567)Find related products by research area.
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| Gene Symbol | MEF2D |
| Uniprot |
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![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- MEF2D used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-NBP1-80567-img0006.jpg "Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- MEF2D used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEF2D, left image and 1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/MEF2D-Antibody-Immunohistochemistry-Paraffin-NBP1-80567-img0005.jpg "Immunohistochemistry-Paraffin: MEF2D Antibody [NBP1-80567] - FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEF2D, left image and 1, right image) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")