Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels ...read more
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of NGFI-B alpha/Nur77/NR4A1 antibody (NB100-56745). The staining was ...read more
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution
NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NGFI-B ...read more
Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - SNRPN downregulated the expression of Nr4a1. Western blot showed the change in the Nr4a1 protein level by SNRPN overexpression in cultured cortical ...read more
RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels of HSPA1B, DNAJB1, IDE, ARC, PER2, PLA2G4E, and NR4A1 proteins ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody
Early response protein NAK1
GFRP1ST-59
growth factor-inducible nuclear protein N10
HMR
HMRNP10
hormone receptor
MGC9485
N10
NAK1
NAK-1
NGFIB alpha
NGFI-B alpha
NGFIB
NR4A1
Nuclear hormone receptor NUR/77
nuclear receptor subfamily 4 group A member 1
nuclear receptor subfamily 4, group A, member 1
Nur77
Orphan nuclear receptor HMR
Orphan nuclear receptor TR3
steroid receptor TR3
Testicular receptor 3
TR3 orphan receptor
TR3
Background
Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Jang S, Zhu J, Qin Q et al. Nur77 Mitigates Endothelial Dysfunction Through Activation of Both Nitric Oxide Production and Anti-Oxidant Pathways SSRN 2023-06-27 (WB, Human)
FAQs for NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745). (Showing 1 - 1 of 1 FAQs).
We have a customer who would like to use NB100-56745 in ChIP experiments. However, they would like to see the data that suggests this antibody could be used in ChIP, which we do not have. Could you pass along that data? Thanks!
Product NB100-56745 is a new product added to our catalog from our recent acquisition with Imgenex. They had verified the use of ChIP with this product from the following publication: p21 expression is induced by activation of nuclear nerve growth factor-induced Ba (Nur77) in pancreatic cancer cells. Lee S-O, S Chintharlapalli, S Liu, S Papineni, SD Cho, K Yoon, S Safe. Mol Cancer Res 7:1169-1178 (2009). IF: Fig 2 (Panc1 cells) WB: Figs 3C and 4B (Panc1 or L3.6pl cells transfected with scrambled or Nur77 siRNA); Figs 6A, 6D (Panc1 cells) ChIP: Fig 5 (Panc1 cells) Note: Nur77 was detected in the nuclear, but not the cytoplasmic fraction of Panc1 cells by WB (Fig 6D). Note: The Nur77 IMG-528 antibody was Nur77 siRNA transfected validated by western blot. Nur77 siRNA, but not scrambled siRNA reduced the intensity of the WB signal in PANC1 and L3.6pi cells (Figs 3C, 4B).
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![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels of NGFI-B alpha/Nur77/NR4A1 protein in three independent replicates, respectively. Dot plots were used for the quantification of the expression levels of protein compared to actin control in WT and calpain-1 KO mice. Unpaired t-test in Prism 7 was used to calculate p values, *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fgene.2020.00334/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-Western-Blot-NB100-56745-img0005.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - RNA-seq results were validated by Western blot analysis in WT and calpain-1 KO mice. Western blot images and quantifications showing the expression levels of NGFI-B alpha/Nur77/NR4A1 protein in three independent replicates, respectively. Dot plots were used for the quantification of the expression levels of protein compared to actin control in WT and calpain-1 KO mice. Unpaired t-test in Prism 7 was used to calculate p values, *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fgene.2020.00334/full), licensed under a CC-BY license.")
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![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-Western-Blot-NB100-56745-img0003.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - Analysis of Nur77 (Nak-1) in 15 ugs of NIH3T3 cell lysate using Nur77 antibody at 1:500 dilution")
![NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/NGFI-B-alpha-Nur77-NR4A1-Antibody-NB100-56745-img0006.jpg "NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with NGFI-B alpha/Nur77/NR4A1 Antibody (NB100-56745) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - SNRPN downregulated the expression of Nr4a1. Western blot showed the change in the Nr4a1 protein level by SNRPN overexpression in cultured cortical neurons. Anti-HA antibody was used to detect the effect of HA-tagged SNRPN plasmid. Anti-SNRPN antibody was used to examine exogenous and endogenous SNRPN proteins, including SmN and SmB. The band about 30 kDa represents exogenous HA-tagged SmN. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27430727/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb100-56745_rabbit-polyclonal-ngfi-b-alpha-nur77-nr4a1-antibody-imgenex-img-528-2092023181155.jpg "Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-56745] - SNRPN downregulated the expression of Nr4a1. Western blot showed the change in the Nr4a1 protein level by SNRPN overexpression in cultured cortical neurons. Anti-HA antibody was used to detect the effect of HA-tagged SNRPN plasmid. Anti-SNRPN antibody was used to examine exogenous and endogenous SNRPN proteins, including SmN and SmB. The band about 30 kDa represents exogenous HA-tagged SmN. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27430727/) licensed under a CC-BY license.")
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