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HDAC5 Antibody - BSA Free

Images

 
Biological Strategies: Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.
Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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HDAC5 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human HDAC5 protein (between residues 1-100) [UniProt Q9UQL6]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 32196931)
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1.0 - 2.0 ug/ml
Application Notes
In Western blot a band was observed ~ 130 kDa. In ICC/IF nuclear staining was observed in A431 cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22152 in the following applications:

Publications
Read Publications using
NBP2-22152 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (Kim et al).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC5 Antibody - BSA Free

  • Antigen NY-CO-9
  • EC 3.5.1.98
  • FLJ90614
  • HD5
  • Histone Deacetylase 5
  • KIAA0600
  • NY-CO-9

Background

HDAC5, or histone deacetylase mHDA1, is responsible for the deacetylation of lysine residues on the N-terminal end of the core histones H2A, H2B, H3 and H4. Identifying histone acetyltransferases reveals much needed insight on how these enzymes regulate transcription, and further elucidates their pivotal role in cell cycle progression and other developmental events. HDAC5 shuttles between the cytoplasm and nucleus, depending on its nuclear export sequence, and shares interactions with BAHD1, KDM5B and MEF family proteins. While HDAC5 has many biological implications, recent studies have proposed its key involvement in synaptic plasticity and memory formation (PMID: 22914591), as well as its connection to various cancers (PMID: 22301920, PMID: 22944197).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC5 Antibody (NBP2-22152)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Chemotaxis, IF/IHC, IHC-P, WB.


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Chemotaxis
(1)
IF/IHC
(1)
IHC-P
(1)
WB
(1)
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Filter By Species
Human
(1)
Mouse
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP2-22152 Applications Species
Laura Lecce, Yang Xu, Bhargavi V’Gangula, Nirupama Chandel, Venu Pothula, Axelle Caudrillier, Maria Paola Santini, Valentina d’Escamard, Delaine K. Ceholski, Przemek A. Gorski, Lijiang Ma, Simon Koplev, Martin Mæng Bjørklund, Johan L.M. Björkegren, Manfred Boehm, Jacob Fog Bentzon, Valentin Fuster, Ha Won Kim, Neal L. Weintraub, Andrew H. Baker, Emily Bernstein, Jason C. Kovacic Histone deacetylase 9 promotes endothelial-mesenchymal transition and an unfavorable atherosclerotic plaque phenotype The Journal of Clinical Investigation 2021-08-02 [PMID: 34338228]
Kohno M, Kobayashi S, Yamamoto T et al. Enhancing calmodulin binding to cardiac ryanodine receptor completely inhibits pressure-overload induced hypertrophic signaling Commun Biol 2020-11-26 [PMID: 33244105] (IF/IHC, Mouse) IF/IHC Mouse
Lee Jo, Byun Ws, Kang Mj et Al. The myokine meteorin-like (metrnl) improves glucose tolerance in both skeletal muscle cells and mice by targeting AMPK alpha 2 FEBS J. 2020-03-20 [PMID: 32196931] (Chemotaxis, Mouse) Chemotaxis Mouse
Tyler C R S, Smoake J J W et al. Sex-Dependent Effects of the Histone Deacetylase Inhibitor, Sodium Valproate, on Reversal Learning After Developmental Arsenic Exposure. Front Genet 2018-03-07 [PMID: 29963072] (WB, Mouse) WB Mouse
Baek MH, Park JY, Rhim CC et al. Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma. Anticancer Res. 2016-05-01 [PMID: 27127168] (IHC-P, Human) IHC-P Human

Review for HDAC5 Antibody (NBP2-22152) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22152:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot HDAC5 NBP2-22152
Enlarge
5
reviewed by:
Liyong Zhang
WB Human 08/15/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for HDAC5 Antibody (NBP2-22152). (Showing 1 - 1 of 1 FAQs).

  1. Do the following antibodies  cross-react with any other the HDAC's? 1) NBP2-22152 and 2) NBP2-16795
    • We have not actually tested these for cross reactivity.   I have looked at homology between he HDAC5/HDAC7 proteins and they are generally only about 60% homologous. As polyclonal antibodies they may cross react slightly.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for HDAC5 Antibody (NBP2-22152)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Liyong Zhang
08/15/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol HDAC5
Entrez
Uniprot