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LIF Antibody [Unconjugated]

 (11 Publications)
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Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND50 is typically 10-60 ng/mL.
Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF449
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF449-SP
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LIF Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse LIF
Specificity
Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than
5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.  
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIF
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Neutralization 10-60 ng/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF449 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody [Unconjugated]

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIF Antibody (AF449)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Bioassay, ELISA Development, ICC, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ELISA Development
(1)
ICC
(1)
Neutralization
(4)
Western Blot
(2)
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Filter By Species
Mouse
(8)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF449 Applications Species
Wei Cai, Ligen Shi, Jingyan Zhao, Fei Xu, Connor Dufort, Qing Ye et al. Neuroprotection against ischemic stroke requires a specific class of early responder T cells in mice Journal of Clinical Investigation 2022-08-01 [PMID: 35912857]
H Lörchner, JM Adrian-Seg, C Waechter, R Wagner, ME Góes, N Brachmann, K Sreenivasa, A Wietelmann, S Günther, N Doll, T Braun, J Pöling Concomitant Activation of OSM and LIF Receptor by a Dual-Specific hlOSM Variant Confers Cardioprotection after Myocardial Infarction in Mice International Journal of Molecular Sciences, 2021-12-29;23(1):. 2021-12-29 [PMID: 35008777] (Western Blot, Mouse) Western Blot Mouse
Giulia Biffi, Tobiloba E. Oni, Benjamin Spielman, Yuan Hao, Ela Elyada, Youngkyu Park et al. IL-1-induced JAK/STAT signaling is antagonized by TGF-beta to shape CAF heterogeneity in pancreatic ductal adenocarcinoma. Cancer Discovery 2019-02-01 [PMID: 30366930]
Y Tang, S Xiong, P Yu, F Liu, L Cheng Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210 Cell Rep, 2018-07-31;24(5):1355-1362.e3. 2018-07-31 [PMID: 30067988] (Bioassay, Mouse) Bioassay Mouse
H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs J. Leukoc. Biol., 2017-05-22;0(0):. 2017-05-22 [PMID: 28533357] (Neutralization, Mouse) Neutralization Mouse
Xin Chen, Bryan S. Hausman, Guangbin Luo, Guang Zhou, Shunichi Murakami, Janet Rubin et al. Protein Kinase Inhibitor gamma Reciprocally Regulates Osteoblast and Adipocyte Differentiation by Downregulating Leukemia Inhibitory Factor Stem Cells 2013-12-01 [PMID: 23963683]
Moidunny S, Vinet J Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity. J Neuroinflammation, 2012-08-16;9(1):198. 2012-08-16 [PMID: 22894638] (ELISA Development, Mouse) ELISA Development Mouse
Quinton LJ, Mizgerd JP, Hilliard KL Leukemia inhibitory factor signaling is required for lung protection during pneumonia. J. Immunol., 2012-05-11;188(12):6300-8. 2012-05-11 [PMID: 22581855] (Neutralization, Mouse) Neutralization Mouse
Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease. Immunity, 2011-08-11;35(2):273-84. 2011-08-11 [PMID: 21835648] (Neutralization, Mouse) Neutralization Mouse
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD Astrocytes promote myelination in response to electrical impulses. Neuron, 2006-03-16;49(6):823-32. 2006-03-16 [PMID: 16543131] (ICC, Western Blot, Mouse) ICC, Western Blot Mouse
Show All 11 Publications.

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Bioinformatics

Gene Symbol LIF