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CCM2 Antibody (OTI1E9)

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Western Blot: CCM2 Antibody (1E9) [NBP2-01134] Analysis of extracts (35ug) from 9 different cell lines by using anti-CCM2 monoclonal antibody.
Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Human lymph node tissue using anti-CCM2 mouse monoclonal antibody.
Western Blot: CCM2 Antibody (1E9) [NBP2-01134] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CCM2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CCM2 Antibody (1E9) [NBP2-01134] - Staining of paraffin-embedded Human lung tissue using anti-CCM2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.38 mg/ml

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CCM2 Antibody (OTI1E9) Summary

Immunogen
Full length human recombinant protein of human CCM2(NP_113631) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCM2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.38 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCM2 Antibody (OTI1E9)

  • C7orf22OSM
  • cerebral cavernous malformation 2
  • Cerebral cavernous malformations 2 protein
  • chromosome 7 open reading frame 22
  • malcavernin
  • MGC4067
  • MGC4607
  • MGC74868

Background

This gene encodes a scaffold protein that functions in the stress-activated p38 Mitogen-activated protein kinase (MAPK) signaling cascade. The protein interacts with SMAD specific E3 ubiquitin protein ligase 1 (also known as SMURF1) via a phosphotyrosine binding domain to promote RhoA degradation. The protein is required for normal cytoskeletal structure, cell-cell interactions, and lumen formation in endothelial cells. Mutations in this gene result in cerebral cavernous malformations. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCM2
Entrez