Oct-3/4 Antibody [Unconjugated] Summary
Immunogen |
E. coli-derived recombinant human Oct‑3/4 Met1-Asn265 (Met262Leu) Accession # Q01860 |
Specificity |
Detects human Oct-3/4 in direct ELISAs and Western blots. |
Source |
N/A |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
POU5F1 |
Purity Statement |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Chromatin Immunoprecipitation (ChIP) 5 ug/10^6 cells
- Immunocytochemistry 5-15 ug/mL
- Immunoprecipitation 3 ug/10^6 cells
- Simple Western 20 ug/mL
- Western Blot 0.5 ug/mL
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Publications |
Read Publications using AF1759 in the following applications:
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Concentration |
LYOPH |
Reconstitution Instructions |
Reconstitute at 0.2 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Oct-3/4 Antibody [Unconjugated]
Background
Oct-3/4, a member of POU transcription factors, was identified as a DNA-binding protein that activates gene transcription via a cis-element containing an octamer motif (1). It is expressed in totipotent embryonic stem and germ cells (2, 3). A critical amount of Oct-3/4 expressed is required to sustain stem cell self-renewal and pluripotency (4). When embryonic stem cells are induced to differentiate, Oct-3/4 is downregulated and this downregulation of Oct-3/4 has proven to be essential for proper and divergent developmental program (5). The Oct-3/4 molecule is not only a master regulator of pluripotency that controls the lineage commitment but also is the most recognized marker used for the identification of totipotent embryonic stem cells.
- Scholer, H.R. et al. (1990) Nature 344:435.
- Scholer, H.R. et al. (1989) EMBO J. 8:2543.
- Rosner, M.H. et al. (1990) Nature 345:686.
- Niwa, H. et al. (2000) Nat. Genet. 24:372.
- Pesce, M. et al. (2001) Stem Cells 19:271.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Oct-3/4 Antibody (AF1759) (0)
Secondary Antibodies
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Isotype Controls
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Additional Oct-3/4 Products
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