Can you confirm whether or not NLS1235C is supported for use in ICC application? Has it been tested in ICC and shown to be ineffective?

Name: Lgr5/GPR49 Antibody [DyLight 650]
Brand: Novus Biologicals
SKU: NLS1235C
Price: 399 USD

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Accepted Answer

Unfortunately, neither the base antibody NLS1235 nor NLS1235C has been tested in ICC/IF. Therefore we don't know whether it will work in ICC. This series of the anybodies with the conjugation formats have only been validated in IHC-P with the human specimens. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing Innovators Reward.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	DyLight 650

Catalog# & Conjugate	Size	Price
Alexa Fluor 405 NLS1235AF405

Alexa Fluor 488 NLS1235AF488

Alexa Fluor 647 NLS1235AF647

Alexa Fluor 700 NLS1235AF700

Biotin NLS1235B

DyLight 405 NLS1235V

DyLight 488 NLS1235G

DyLight 550 NLS1235R

FITC NLS1235F

Unconjugated NLS1235

Catalog# & Formulation	Size	Price
Standard Formulation NLS1235

Azide and BSA Free NLS1235SS

Azide and BSA Free NLS1235

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 405	NLS1235AF405
Alexa Fluor 488	NLS1235AF488
Alexa Fluor 647	NLS1235AF647
Alexa Fluor 700	NLS1235AF700
Biotin	NLS1235B
DyLight 405	NLS1235V
DyLight 488	NLS1235G
DyLight 550	NLS1235R
FITC	NLS1235F
Unconjugated	NLS1235

Lgr5/GPR49 Antibody [DyLight 650]

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Bioinformatics

Immunogen	Synthetic peptide [KLH conjugated] made to the 2nd cytoplasmic loop of human GPR49/LGR5. [UniProt# O75473]
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	LGR5
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Dilutions	Immunohistochemistry 5-8 ug/ml Immunohistochemistry-Paraffin 5-8 ug/ml
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified