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CD133 Antibody - BSA Free

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Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining ...read more
Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals ...read more
Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained ...read more
Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

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CD133 Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 1:500-1:1000
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Publications
Read Publications using
NB120-16518 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD133 Antibody - BSA Free

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ELISA, Flow, ICC/IF, IHC, ChIP

Publications for CD133 Antibody (NB120-16518)(42)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 11 applications: B/N, ELISA, FLOW, Flow Cytometry, ICC/IF, IF/IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, WB.


Filter By Application
B/N
(1)
ELISA
(1)
FLOW
(9)
Flow Cytometry
(1)
ICC/IF
(8)
IF/IHC
(6)
IHC-Fr
(1)
IHC-P
(2)
Immunocytochemistry/ Immunofluorescence
(3)
Immunohistochemistry-Paraffin
(1)
WB
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(3)
Rat
(5)
Porcine
(2)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB120-16518 Applications Species
Depanwita Saha, Debarpan Mitra, Neyaz Alam, Sagar Sen, Saunak Mitra Mustafi, Pradip K Majumder, Biswanath Majumder, Nabendu Murmu Lupeol and Paclitaxel cooperate in hindering hypoxia induced vasculogenic mimicry via suppression of HIF-1 alpha -EphA2-Laminin-5 gamma 2 network in human oral cancer. Journal of cell communication and signaling 2023-09-01 [PMID: 36063341]
Zhou L, Yang T, Zhao F Et al. Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats Sci Rep 2022-03-05 [PMID: 35246575] (FLOW, Rat)

Details:
Citation using the PE version of this antibody.
FLOW Rat
King P, Wan J, Guo AA et al. Regulation of gliomagenesis and stemness through acid sensor ASIC1a International journal of oncology 2021-10-01 [PMID: 34515325] (FLOW, Human) FLOW Human
Madlala SC The role of stem cells and WNT signalling pathway in renal cell carcinoma Thesis 2020-01-01 (IHC-P, Human) IHC-P Human
Pomares, A Tumorspheres as an in vitro model for cancer stem-like cell characterization in non-small cell lung cancer. Prognostic implications. Thesis 2020-01-01 (ICC/IF) ICC/IF
Tarek Niemann, Jonas Joneleit, Jonathan Storm, Tom Nacke, Dirk Wähnert, Christian Kaltschmidt, Thomas Vordemvenne, Barbara Kaltschmidt, Kin Hing William Lau, Tong-Chuan He Analyzing Sex-Specific Dimorphism in Human Skeletal Stem Cells Cells 2023-11-22 [PMID: 38067111]
Iqbal F, Johnston A, Wyse B et al. Combination human umbilical cord perivascular and endothelial colony forming cell therapy for ischemic cardiac injury npj Regenerative Medicine 2023-08-25 [PMID: 37626067] (Flow Cytometry) Flow Cytometry
Wu MH, Wu K, Zhu YB et al. Baicalin Antagonizes Prostate Cancer Stemness via Inhibiting Notch1/NF-?B Signaling Pathway Chinese journal of integrative medicine 2023-06-26 [PMID: 37357241]
Filidou E, Kandilogiannakis L, Tarapatzi G et al. A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Biomedicines 2023-03-05 [PMID: 36979766] (Immunocytochemistry/ Immunofluorescence, Human) Immunocytochemistry/ Immunofluorescence Human
Andreas M. Bapst, Thomas Knöpfel, Karen A. Nolan, Faik Imeri, Claus D. Schuh, Andrew M. Hall, Jia Guo, Dörthe M. Katschinski, Roland H. Wenger Neurogenic and pericytic plasticity of conditionally immortalized cells derived from renal erythropoietin‐producing cells Journal of Cellular Physiology 2022-01-10 [PMID: 35014036]
Show All 42 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for CD133 Antibody (NB120-16518)

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1