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Musashi-1 Antibody [Unconjugated]

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Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Musashi-1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows 25 ng of Recombinant Human Musashi-1 and Recombinant Human Musashi-2. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody ...read more
Musashi-1 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at ...read more
Musashi-1 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) at 10 µg/mL for 3 hours at ...read more
Musashi-1 was detected in immersion fixed paraffin-embedded sections of human intestine using 1 µg/mL Goat Anti-Human/Mouse/ Rat Musashi-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2628) overnight at 4 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2628-SP
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Musashi-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Musashi-1
Met1-His362
Accession # O43347
Specificity
Detects human Musashi-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Musashi‑2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MSI1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2628 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Musashi-1 Antibody [Unconjugated]

  • MSI1
  • Musashi (Drosophila) homolog 1
  • musashi homolog 1 (Drosophila)
  • Musashi1
  • Musashi-1
  • RNA-binding protein Musashi homolog 1

Background

Musashi-1 is an RNA-binding protein that is expressed in the developing central nervous system. Expression is highest in proliferating multipotent neural precursor cells and decreases during neuronal differentiation (1, 2).
  1. Kaneko, Y. et al. (2000) Dev. Neurosci. 22:139.
  2. Kanemura, Y. et al. (2002) Meth. Mol. Biol. 198:273.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Musashi-1 Antibody
[Unconjugated]
AF2628
Musashi-1 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC/IF

Publications for Musashi-1 Antibody (AF2628)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 3 applications: ICC, IHC, Immunohistochemistry.


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ICC
(3)
IHC
(1)
Immunohistochemistry
(1)
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Human
(3)
Mouse
(1)
Transgenic Mouse
(1)
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Showing Publications 1 - 7 of 7.
Publications using AF2628 Applications Species
Bychkov, I;Deneka, A;Topchu, I;Pangeni, R;Ismail, A;Lengner, C;Karanicolas, J;Golemis, E;Makhov, P;Boumber, Y; Musashi-2 (MSI2) regulation of DNA damage response in lung cancer. Research square 2024-04-11 [PMID: 38659828] (Immunohistochemistry, Transgenic Mouse) Immunohistochemistry Transgenic Mouse
Bychkov, I;Deneka, A;Topchu, I;Pangeni, RP;Lengner, C;Karanicolas, J;Golemis, EA;Makhov, P;Boumber, Y; Musashi-2 (MSI2) regulation of DNA damage response in lung cancer bioRxiv : the preprint server for biology 2023-06-14 [PMID: 37398283] (IHC, Mouse) IHC Mouse
Jonathan Stefanowski, Annemarie Lang, Ariana Rauch, Linus Aulich, Markus Köhler, Alexander F. Fiedler et al. Spatial Distribution of Macrophages During Callus Formation and Maturation Reveals Close Crosstalk Between Macrophages and Newly Forming Vessels Frontiers in Immunology 2019-11-26 [PMID: 31956322]
DS Kim, SJ Jung, JS Lee, BY Lim, HA Kim, JE Yoo, DW Kim, JW Leem Rapid generation of OPC-like cells from human pluripotent stem cells for treating spinal cord injury Exp. Mol. Med., 2017-07-28;49(7):e361. 2017-07-28 [PMID: 28751784] (ICC, Human) ICC Human
Jessie M. Sutherland, Alexander P. Sobinoff, Kara M. Gunter, Barbara A. Fraser, Victoria Pye, Ilana R. Bernstein et al. Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis Biomolecules 2015-06-26 [PMID: 26131972]
Jagtap , Jayashre, Dawood , Parveen, Shah , Reecha D, Chandrika , Goparaju, Natesh , Kumar, Shiras , Anjali, Hegde , Amba S, Ranade , Deepak, Shastry , Padma Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis. PLoS ONE, 2014-02-11;9(2):e88505. 2014-02-11 [PMID: 24523904] (ICC, Human) ICC Human
Burkert J, Otto WR, Wright NA Side populations of gastrointestinal cancers are not enriched in stem cells. J. Pathol., 2008-04-01;214(5):564-73. 2008-04-01 [PMID: 18266310] (ICC, Human) ICC Human

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Bioinformatics

Gene Symbol MSI1
Uniprot