Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11). Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.

Showing Publications 1 - 9 of 9.

Publications using 7150-RS/CF	Applications	Species
A Hiroto, WK Kim, A Pineda, Y He, DH Lee, V Le, AW Olson, J Aldahl, CH Nenninger, AJ Buckley, GQ Xiao, J Geradts, Z Sun Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis Nature Communications, 2022-11-02;13(1):6552. 2022-11-02 [PMID: 36323713] (Bioassay, Mouse)	Bioassay	Mouse
Z Fu, JW Dean, L Xiong, MW Dougherty, KN Oliff, ZE Chen, C Jobin, TJ Garrett, L Zhou Mitochondrial transcription factor A in RORgammat+ lymphocytes regulate small intestine homeostasis and metabolism Nature Communications, 2021-07-22;12(1):4462. 2021-07-22 [PMID: 34294718] (Bioassay, Mouse)	Bioassay	Mouse
K Dunbar, A Valanciute, A Lima, PF Vinuela, T Jamieson, V Rajasekara, J Blackmur, AM Ochocka-Fo, A Guazzelli, P Cammareri, MJ Arends, OJ Sansom, KB Myant, SM Farrington, MG Dunlop, FVN Din Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1 Cell Mol Gastroenterol Hepatol, 2020-09-22;0(0):. 2020-09-22 [PMID: 32971322] (Cell Culture, Mouse)	Cell Culture	Mouse
MW Dougherty, O Kudin, M Mühlbauer, J Neu, RZ Gharaibeh, C Jobin Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites BMC Microbiol., 2020-07-11;20(1):205. 2020-07-11 [PMID: 32652929] (Bioassay, Mouse)	Bioassay	Mouse
Y Cai, G Zhu, S Liu, Z Pan, M Quintero, CJ Poole, C Lu, H Zhu, B Islam, JV Riggelen, D Browning, K Liu, R Blumberg, N Singh, H Li Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation Cell Discov, 2019-01-29;5(0):7. 2019-01-29 [PMID: 30701081] (Bioassay, Mouse)	Bioassay	Mouse
Y Eriguchi, K Nakamura, Y Yokoi, R Sugimoto, S Takahashi, D Hashimoto, T Teshima, T Ayabe, ME Selsted, AJ Ouellette Essential role of IFN-? in T cell-associated intestinal inflammation JCI Insight, 2018-09-20;3(18):. 2018-09-20 [PMID: 30232288] (Bioassay, Mouse)	Bioassay	Mouse
G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo Proc. Natl. Acad. Sci. U.S.A., 2018-03-20;0(0):. 2018-03-20 [PMID: 29559533] (Bioassay, Human)	Bioassay	Human
X Yin, H Yi, L Wang, W Wu, X Wu, L Yu RSPOs facilitated HSC activation and promoted hepatic fibrogenesis Oncotarget, 2016-09-27;7(39):63767-63778. 2016-09-27 [PMID: 27572318] (Bioassay, Mouse)	Bioassay	Mouse
Marie-Isabelle Garcia Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage Development, 2016-03-17;143(9):1452-63. 2016-03-17 [PMID: 26989172] (Bioassay, Mouse)	Bioassay	Mouse

Array 7150-RS/CF

• R-Spondin 1 Antibodies
• R-Spondin 1 Lysate
• R-Spondin 1 Proteins
• R-Spondin 1 Proteins and Enzymes
• R-Spondin 1 ELISA