Name: ALCAM/CD166 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF1172
Rating: 4.5 (2 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Mu, Rt, CaSpecies Glossary
Applications	WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 700 FAB1172N-100UG

Allophycocyanin FAB1172A

Biotin BAF1172

Fluorescein FAB1172F

Phycoerythrin FAB1172P

Catalog# & Formulation	Size	Price
Carrier Free FAB1172N-100UG

Carrier Free FAB1172P

Carrier Free FAB1172A

Carrier Free FAB1172F

Carrier Free AF1172-SP

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 700	FAB1172N-100UG
Allophycocyanin	FAB1172A
Biotin	BAF1172
Fluorescein	FAB1172F
Phycoerythrin	FAB1172P

ALCAM/CD166 Antibody [Unconjugated]

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Publications for ALCAM/CD166 Antibody (AF1172)(22)

Reviews for ALCAM/CD166 Antibody (AF1172) (2) 4.52

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Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166 Trp28-Lys527 Accession # AAC06342
Specificity	Detects human, mouse and rat ALCAM/CD166 in Western blots. In direct ELISAs, less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed. Detects canine ALCAM/CD166 in flow cytometry.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	ALCAM
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Publications using AF1172	Applications	Species
Mapunda JA, Pareja J, Vladymyrov M et al. VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammation Nat Commun 2023-09-20 [PMID: 37730744]
Robin W. Yeo, Olivia Y. Zhou, Brian L. Zhong, Eric D. Sun, Paloma Navarro Negredo, Surag Nair et al. Chromatin accessibility dynamics of neurogenic niche cells reveal defects in neural stem cell adhesion and migration during aging Nature Aging 7/13/2023 [PMID: 37443352]
S He, S Li, J Guo, X Zeng, D Liang, Y Zhu, Y Li, D Yang, X Zhao CD166-specific CAR-T cells potently target colorectal cancer cells Translational Oncology, 2022-10-31;27(0):101575. 2022-10-31 [PMID: 36327697] (Flow Cytometry, ICC, Western Blot, Human)	Flow Cytometry, ICC, Western Blot	Human
Camila Delgado, Lei Bu, Jie Zhang, Fang-Yu Liu, Joseph Sall, Feng-Xia Liang et al. Neural cell adhesion molecule is required for ventricular conduction system development Development 2021-06-01 [PMID: 34100064]
Hiromi Yamashita, Keiichi Fukuda, and Fumiyuki Hattori Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells Can Be Enriched by a Combination of Mitochondrial Content and Activated Leukocyte Cell Adhesion Molecule Japan Medical Association Journal 2019-09-04 [PMID: 33615028] (IHC, Flow Cytometry, Human, Mouse)	IHC, Flow Cytometry	Human, Mouse
AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church A comprehensive library of human transcription factors for cell fate engineering Nature Biotechnology, 2020-11-30;0(0):. 2020-11-30 [PMID: 33257861] (ICC, Human)	ICC	Human
HF Renard, F Tyckaert, C Lo Giudice, T Hirsch, CA Valades-Cr, C Lemaigre, M Shafaq-Zad, C Wunder, R Wattiez, L Johannes, P van der Br, D Alsteens, P Morsomme Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166 Nat Commun, 2020-03-19;11(1):1457. 2020-03-19 [PMID: 32193381] (IF, Mouse)	IF	Mouse
CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 2019-07-12;0(0):. 2019-07-12 [PMID: 31300520] (IHC, Mouse)	IHC	Mouse
Ann-Helen Willrodt, Ann-Charlott Salabarria, Philipp Schineis, Desislava Ignatova, Morgan Campbell Hunter, Martina Vranova et al. ALCAM Mediates DC Migration Through Afferent Lymphatics and Promotes Allospecific Immune Reactions Frontiers in Immunology 2019-04-12 [PMID: 31031759]
Ubadah Sabbagh, Aboozar Monavarfeshani, Kaiwen Su, Masoud Zabet‐Moghadam, James Cole, Eric Carnival et al. Distribution and development of molecularly distinct perineuronal nets in visual thalamus Journal of Neurochemistry 2018-12-01 [PMID: 30326149]
J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018-04-09;0(0):. 2018-04-09 [PMID: 29632360] (IHC, Mouse)	IHC	Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)	IHC	Mouse
Nicholas R. Smith, Paige S. Davies, Trevor G. Levin, Alexandra C. Gallagher, Douglas R. Keene, Sidharth K. Sengupta et al. Cell Adhesion Molecule CD166/ALCAM Functions Within the Crypt to Orchestrate Murine Intestinal Stem Cell Homeostasis Cellular and Molecular Gastroenterology and Hepatology 2017-05-01 [PMID: 28462380]
MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E524-E533. 2017-01-09 [PMID: 28069965] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Gu , Zirong, Imai , Fumiyasu, Kim , In Jung, Fujita , Hiroko, Katayama , Kei ichi, Mori , Kensaku, Yoshihara , Yoshihir, Yoshida , Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015-03-31;10(3):e0121550. 2015-03-31 [PMID: 25826454] (IHC, Mouse)	IHC	Mouse
Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015-03-09;112(15):E1946-55. 2015-03-09 [PMID: 25775569] (Flow Cytometry, IHC, Mouse, Rat)	Flow Cytometry, IHC	Mouse, Rat
Leibowitz B, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T, Yu J Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice. Nat Commun, 2014-03-18;5(0):3494. 2014-03-18 [PMID: 24637717] (IHC, Mouse)	IHC	Mouse
Inamata , Yasuyuki, Shirasaki , Ryuichi Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axons. Development, 2014-02-19;141(6):1260-71. 2014-02-19 [PMID: 24553291] (IHC, Mouse)	IHC	Mouse
Rüdiger von Bauer, Dimitrios Oikonomou, Alba Sulaj, Sawsan Mohammed, Agnes Hotz-Wagenblatt, Hermann-Josef Gröne et al. CD166/ALCAM Mediates Proinflammatory Effects of S100B in Delayed Type Hypersensitivity The Journal of Immunology 2013-07-01 [PMID: 23729438]
Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. J. Neurosci., 2011-04-20;31(16):5942-55. 2011-04-20 [PMID: 21508219] (IHC-P, Human)	IHC-P	Human
Di Meglio T, Nguyen-Ba-Charvet KT, Tessier-Lavigne M, Sotelo C, Chedotal A Molecular mechanisms controlling midline crossing by precerebellar neurons. J. Neurosci., 2008-06-18;28(25):6285-94. 2008-06-18 [PMID: 18562598] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Stanton LA, Beier F Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells. Exp. Cell Res., 2006-10-04;313(1):146-55. 2006-10-04 [PMID: 17067574] (ICC, Mouse)	ICC	Mouse
Show All 22 Publications. Collapse Publications.

	Verified Customer 01/05/2015
Application:	IF
Species:	Mouse

	Verified Customer 01/05/2015
Application:	IF
Species:	Mouse

Gene Symbol	ALCAM
Uniprot	AAC06342()