![Immunocytochemistry/Immunofluorescence: CD24 Antibody (ML5) [NB100-77903] - MCF-7 breast cancer cells.](http://images.novusbio.com/fullsize/CD24-Antibody-ML5-Immunocytochemistry-Immunofluorescence-NB100-77903-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD24 Antibody (ML5) [NB100-77903] - MCF-7 breast cancer cells.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | ML5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen was the full length version of the protein: CD24 (ML5) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD24 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NB100-77903 | Applications | Species |
|---|---|---|
| Zhang J, Nguyen LTH, Hickey R et al. Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids Scientific reports 2021-04-13 [PMID: 33850163] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for CD24 Antibody (NB100-77903)Find related products by research area.
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Tools for Isolation, Quantification and Analysis of Exosomes Exosomes are spherical to cup-shaped bilayered membrane enclosed nanosize vesicles (30-100 nm) which have the ability to shuttle active cargoes between cells. Johnstone et al. 1987 pioneered in documenting the generation of exosomes in differentiat... Read full blog post. |
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![Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.](http://images.novusbio.com/fullsize/CD24-Antibody-ML5-Flow-Cytometry-NB100-77903-img0004.jpg "Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.")
![Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC](http://images.novusbio.com/fullsize/CD24-Antibody-ML5-Flow-Cytometry-NB100-77903-img0001.jpg "Flow Cytometry: CD24 Antibody (ML5) [NB100-77903] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC")
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