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Lgr5/GPR49 Antibody [Alexa Fluor® 647]

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Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on HepG2 cells with Lgr5/GPR49 Antibody NBP1-28904AF647 (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (M2-7C10) NBP1-28904AF647 (blue) and a matched isotype ...read more
Flow Cytometry: Lgr5/GPR49 Antibody [Alexa Fluor® 647] [NBP1-28904AF647] - An intracellular stain was performed on SH-SY5Y cells with Lgr5/GPR49 Antibody [13A9] NBP1-28904AF647 (blue) and a matched isotype control ...read more

Product Details

Summary
Reactivity Hu, Mu, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 647

Order Details

Lgr5/GPR49 Antibody [Alexa Fluor® 647] Summary

Immunogen
Synthetic peptide made to an internal portion of the human GPR49/LGR5 protein (within residues 650-700). [Swiss-Prot: O75473]
Localization
Cell-membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LGR5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32016468).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lgr5/GPR49 Antibody [Alexa Fluor® 647]

  • FEX
  • G protein-coupled receptor 49
  • GPR49
  • GPR49G-protein coupled receptor HG38
  • GPR67
  • G-protein coupled receptor 49
  • G-protein coupled receptor 67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G protein-coupled receptor 5
  • leucine-rich repeat-containing G-protein coupled receptor 5
  • Lgr5
  • MGC117008
  • orphan G protein-coupled receptor HG38

Background

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Lgr5/GPR49.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

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Bioinformatics

Gene Symbol LGR5