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JunB/AP-1 Antibody (3U10K1)

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Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Western blot analysis of extracts of BxPC-3 cells, using JunB/AP-1 Rabbit mAb (NBP3-16491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Immunohistochemistry of paraffin-embedded human esophageal using JunB/AP-1 Rabbit mAb (NBP3-16491) at dilution of 1:100 (40x lens).Perform ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
3U10K1
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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JunB/AP-1 Antibody (3U10K1) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JunB/AP-1 (P17275). MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVIT
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
JUNB
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for JunB/AP-1 Antibody (3U10K1)

  • activator protein 1
  • AP-1
  • jun B proto-oncogene
  • JunB
  • transcription factor jun-B

Background

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol JUNB